Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17201-1-AP targets PLXNA4 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10978 Product name: Recombinant human PLXNA4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-358 aa of BC028744 Sequence: MKAMPWNWTCLLSHLLMVGMGSSTLLTRQPAPLSQKQRSFVTFRGEPAEGFNHLVVDERTGHIYLGAVNRIYKLSSDLKVLVTHETGPDEDNPKCYPPRIVQTCNEPLTTTNNVNKMLLIDYKENRLIACGSLYQGICKLLRLEDLFKLGEPYHKKEHYLSGVNESGSVFGVIVSYSNLDDKLFIATAVDGKPEYFPTISSRKLTKNSEADGMFAYVFHDEFVASMIKIPSDTFTIIPDFDIYYVYGFSSGNFVYFLTLQPEMVSPPGSTTKEQVYTSKLVRLCKEDTAFNSYVEVPIGCERSGVEYRLLQAAYLSKAGAVLGRTLGVHPDDDLLFTVFSKGQKRKMKSLDESALCIF 相同性解析による交差性が予測される生物種 |
| Full Name | plexin A4 |
| Calculated molecular weight | 522aa,58 kDa; 1894aa,212 kDa |
| Observed molecular weight | 212 kDa |
| GenBank accession number | BC028744 |
| Gene Symbol | PLXNA4 |
| Gene ID (NCBI) | 91584 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9HCM2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PLXNA4 is a plexin family receptor that transduces semaphorin signals to regulate axon guidance, vascular development, and immune cell migration. It functions as a plasma membrane receptor with intracellular GTPase-regulatory activity, linking extracellular cues to cytoskeletal remodeling. Dysregulation of PLXNA4 has been associated with neurological disease (including Alzheimer's), cancer progression, and vascular/immune dysfunction, making it a multifunctional receptor of significant biomedical interest. (PMID: 27127761)
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for PLXNA4 antibody 17201-1-AP | Download protocol |
| WB protocol for PLXNA4 antibody 17201-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |