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Validation Data Gallery

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  • Western Blot (WB) analysis of MKN-45 cells using PLXDC2 Recombinant antibody (83449-4-RR)

    WB analysis of MKN-45 using 83449-4-RR (same clone as 83449-4-PBS)

    MKN-45 cells were subjected to SDS PAGE followed by western blot with 83449-4-RR (PLXDC2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83449-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using PLXDC2 Recombinant antibody (83449-4-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 83449-4-RR (same clone as 83449-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83449-4-RR (PLXDC2 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83449-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using PLXDC2 Recombinant antibody (83449-4-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 83449-4-RR (same clone as 83449-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83449-4-RR (PLXDC2 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83449-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83449-4-RR

    Affinity and Kinetic Characterization of 83449-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83449-4-RR against Human PLXDC2 were performed. The affinity constant is 42.5 pM.

PLXDC2 Recombinant monoclonal antibody, PBS Only

PLXDC2 Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 83449-4-PBS
Clone No.240174A11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

UNQ2514/PRO6003, Tumor endothelial marker 7-related protein, TEM7R, Plexin domain-containing protein 2, plexin domain containing 2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83449-4-PBS targets PLXDC2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag2940

Product name: Recombinant human PLXDC2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 28-407 aa of BC012885

Sequence: ADGKPGDQILDWQYGVTQAFPHTEEEVEVDSHAYSHRWKRNLDFLKAVDTNRASVGQDSPEPRSFTDLLLDDGQDNNTQIERVNLSFDFPFYGHFLREITVATGGFIYTGEVVHRMLTATQYIAPLMANFDPSVSRNSTVRYFDNGTALVVQWDHVHLQDNYNLGSFTFQATLLMDGRIIFGYKEIPVLVTQISSTNHPVKVGLSDAFVVVHRIQQIPNVRRRTIYEYHRVELQMSKITNISAVEMTPLPTCLQFNRCGPCVSSQIGFNCSWCSKLQRCSSGFDRHRQDWVDSGCPEESKEKMCENTEPVETSSRTTTTVGATTTQFRVLTTTRRAVTSQFPTSLPTEDDTKIALHLKDNGASTDDSAAEKKGGTLHAGL

相同性解析による交差性が予測される生物種
Full Name plexin domain containing 2
Calculated molecular weight 480 aa, 54 kDa
Observed molecular weight 54 kDa
GenBank accession numberBC012885
Gene Symbol PLXDC2
Gene ID (NCBI) 84898
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ6UX71
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PLXDC2 (Plexin-domain containing 2), also named tumor endothelial marker 7-related protein (TEM7R), is a single-pass type I membrane protein with some homology to nidogen and to plexins. PLXDC2 is located in tumor endothelium and plays a role in tumor angiogenesis. Three isoforms of PLXDC2 produced by alternative splicing have been described. Recent finding revealed that PLXDC2 is a mitogen for neural progenitors and functions as a novel regulator of the development of different brain regions, suggesting its role in coordinating proliferation and differentiation in the developing nervous system.

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