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Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using PLXDC2 Polyclonal antibody (12285-1-AP)

    WB analysis of A431 using 12285-1-AP (same clone as 12285-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 12285-1-AP (PLXDC2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12285-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PLXDC2 Polyclonal antibody (12285-1-AP)

    WB analysis using 12285-1-AP (same clone as 12285-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12285-1-AP (PLXDC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12285-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using PLXDC2 Polyclonal antibody (12285-1-AP)

    WB analysis of HeLa using 12285-1-AP (same clone as 12285-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 12285-1-AP (PLXDC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12285-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PLXDC2 Polyclonal antibody (12285-1-AP)

    IHC staining of human lung cancer using 12285-1-AP (same clone as 12285-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12285-1-AP (PLXDC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12285-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PLXDC2 Polyclonal antibody (12285-1-AP)

    IHC staining of human lung cancer using 12285-1-AP (same clone as 12285-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12285-1-AP (PLXDC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12285-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PLXDC2 Polyclonal antibody (12285-1-AP)

    IHC staining of human liver cancer using 12285-1-AP (same clone as 12285-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12285-1-AP (PLXDC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12285-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PLXDC2 Polyclonal antibody (12285-1-AP)

    IHC staining of human liver cancer using 12285-1-AP (same clone as 12285-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12285-1-AP (PLXDC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12285-1-PBS in a different storage buffer formulation.

PLXDC2 Polyclonal antibody, PBS Only

PLXDC2 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 12285-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

UNQ2514/PRO6003, Tumor endothelial marker 7-related protein, TEM7R, Plexin domain-containing protein 2, plexin domain containing 2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12285-1-PBS targets PLXDC2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2940

Product name: Recombinant human PLXDC2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 28-407 aa of BC012885

Sequence: ADGKPGDQILDWQYGVTQAFPHTEEEVEVDSHAYSHRWKRNLDFLKAVDTNRASVGQDSPEPRSFTDLLLDDGQDNNTQIERVNLSFDFPFYGHFLREITVATGGFIYTGEVVHRMLTATQYIAPLMANFDPSVSRNSTVRYFDNGTALVVQWDHVHLQDNYNLGSFTFQATLLMDGRIIFGYKEIPVLVTQISSTNHPVKVGLSDAFVVVHRIQQIPNVRRRTIYEYHRVELQMSKITNISAVEMTPLPTCLQFNRCGPCVSSQIGFNCSWCSKLQRCSSGFDRHRQDWVDSGCPEESKEKMCENTEPVETSSRTTTTVGATTTQFRVLTTTRRAVTSQFPTSLPTEDDTKIALHLKDNGASTDDSAAEKKGGTLHAGL

相同性解析による交差性が予測される生物種
Full Name plexin domain containing 2
Calculated molecular weight 480 aa, 54 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC012885
Gene Symbol PLXDC2
Gene ID (NCBI) 84898
RRIDAB_2166239
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6UX71
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PLXDC2 (Plexin-domain containing 2), also named tumor endothelial marker 7-related protein (TEM7R), is a single-pass type I membrane protein with some homology to nidogen and to plexins. PLXDC2 is located in tumor endothelium and plays a role in tumor angiogenesis. Three isoforms of PLXDC2 produced by alternative splicing have been described. Recent finding revealed that PLXDC2 is a mitogen for neural progenitors and functions as a novel regulator of the development of different brain regions, suggesting its role in coordinating proliferation and differentiation in the developing nervous system.

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