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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse lung tissue using PLUNC Polyclonal antibody (10413-1-AP)

    WB analysis of mouse lung using 10413-1-AP (same clone as 10413-1-PBS)

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 10413-1-AP (PLUNC antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10413-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse lung tissue using PLUNC Polyclonal antibody (10413-1-AP)

    IP experiment of mouse lung using 10413-1-AP (same clone as 10413-1-PBS)

    IP result of anti-PLUNC (IP:10413-1-AP, 4ug; Detection:10413-1-AP 1:300) with mouse lung tissue lysate 3600ug. This data was developed using the same antibody clone with 10413-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using PLUNC Polyclonal antibody (10413-1-AP)

    IHC staining of mouse lung using 10413-1-AP (same clone as 10413-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 10413-1-AP (PLUNC antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10413-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using PLUNC Polyclonal antibody (10413-1-AP)

    IHC staining of mouse lung using 10413-1-AP (same clone as 10413-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 10413-1-AP (PLUNC antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10413-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PLUNC Polyclonal antibody (10413-1-AP)

    IHC staining of human lung cancer using 10413-1-AP (same clone as 10413-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10413-1-AP (PLUNC Antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 10413-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PLUNC Polyclonal antibody (10413-1-AP)

    IHC staining of human lung cancer using 10413-1-AP (same clone as 10413-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10413-1-AP (PLUNC Antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 10413-1-PBS in a different storage buffer formulation.

PLUNC Polyclonal antibody, PBS Only

PLUNC Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 10413-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

BPI fold-containing family A member 1, BPIFA1, Lung-specific protein X, LUNX, NASG

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10413-1-PBS targets PLUNC in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag0629

Product name: Recombinant human PLUNC protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-256 aa of BC012549

Sequence: MFQTGGLIVFYGLLAQTMAQFGGLPVPLDQTLPLNVNPALPLSPTGLAGSLTNALSNGLLSGGLLGILENLPLLDILKPGGGTSGGLLGGLLGKVTSVIPGLNNIIDIKVTDPQLLELGLVQSPDGHRLYVTIPLGIKLQVNTPLVGASLLRLAVKLDITAEILAVRDKQERIHLVLGDCTHSPGSLQISLLDGLGPLPIQGLLDSLTGILNKVLPELVQGNVCPLVNEVLRGLDITLVHDIVNMLIHGLQFVIKV

相同性解析による交差性が予測される生物種
Full Name palate, lung and nasal epithelium associated
Calculated molecular weight 27 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC012549
Gene Symbol PLUNC
Gene ID (NCBI) 51297
RRIDAB_2166093
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NP55
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PLUNC, also named as LUNX, NASG and SPURT, belongs to the BPI/LBP/Plunc superfamily and Plunc family. PLUNC may be involved in the airway inflammatory response after exposure to irritants. It is associated with tumor progression. PLUNC plays a role in innate immune responses of the upper airways.

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