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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PLTP Recombinant antibody (84592-1-RR)

    WB analysis using 84592-1-RR (same clone as 84592-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84592-1-RR (PLTP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84592-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PLTP Recombinant antibody (84592-1-RR)

    IHC staining of mouse brain using 84592-1-RR (same clone as 84592-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84592-1-RR (PLTP antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84592-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PLTP Recombinant antibody (84592-1-RR)

    IHC staining of mouse brain using 84592-1-RR (same clone as 84592-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84592-1-RR (PLTP antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84592-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using PLTP Recombinant antibody (84592-1-RR)

    IF Staining of mouse brain using 84592-1-RR (same clone as 84592-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using PLTP antibody (84592-1-RR, Clone: 241583F3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84592-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84592-1-RR

    Affinity and Kinetic Characterization of 84592-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84592-1-RR against Human PLTP were performed. The affinity constant is below 1 pM.

PLTP Recombinant monoclonal antibody, PBS Only

PLTP Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 84592-1-PBS
Clone No.241583F3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

241583F3, Lipid transfer protein II, Phospholipid transfer protein

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84592-1-PBS targets PLTP in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag8171

Product name: Recombinant human PLTP protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 163-441 aa of BC005045

Sequence: IDYSLMKDPVASTSNLDMDFRGAFFPLTERNWSLPNRAVEPQLQEEERMVYVAFSEFFFDSAMESYFRAGALQLLLVGDKVPHDLDMLLRATYFGSIVLLSPAVIDSPLKLELRVLAPPRCTIKPSGTTISVTASVTIALVPPDQPEVQLSSMTMDARLSAKMALRGKALRTQLDLRRFRIYSNHSALESLALIPLQAPLKTMLQIGVMPMLNERTWRGVQIPLPEGINFVHEVVTNHAGFLTIGADLHFAKGLREVIEKNRPADVRASTAPTPSTAAV

相同性解析による交差性が予測される生物種
Full Name phospholipid transfer protein
Calculated molecular weight 493 aa, 55 kDa
Observed molecular weight55 kDa
GenBank accession numberBC005045
Gene Symbol PLTP
Gene ID (NCBI) 5360
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDP55058
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PLTP (phospholipid transfer protein) belongs to the family of lipid-binding and lipid-transfer proteins. PLTP is involved in the distribution and metabolism of high-density lipoprotein ( HDL ) and reverse cholesterol transfer ( RCT ). In plasma, PLTP catalyzes the transfer of phospholipids ( mainly phosphatidylcholine ) between lipoproteins (PMID: 29558025). PLTP has a predicted protein molecular mass of approximately 55 kDa, and molecular weights of 70-80 kDa can also be detected in some literature (PMID: 26160914; 36312255; 29680823; 15342679; 7654777).

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