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Validation Data Gallery

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  • Western Blot (WB) analysis of human heart tissue using PLSCR4 Polyclonal antibody (12630-1-AP)

    WB analysis of human heart using 12630-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 12630-1-AP (PLSCR4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using PLSCR4 Polyclonal antibody (12630-1-AP)

    WB analysis of human brain using 12630-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12630-1-AP (PLSCR4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using PLSCR4 Polyclonal antibody (12630-1-AP)

    WB analysis of mouse testis using 12630-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 12630-1-AP (PLSCR4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using PLSCR4 Polyclonal antibody (12630-1-AP)

    WB analysis of human placenta using 12630-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 12630-1-AP (PLSCR4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using PLSCR4 Polyclonal antibody (12630-1-AP)

    WB analysis of human liver using 12630-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 12630-1-AP (PLSCR4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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PLSCR4 Polyclonal antibody

PLSCR4 Polyclonal Antibody for WB, ELISA
Cat No. 12630-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

phospholipid scramblase 4, PL scramblase 4, PLSCR4, TRA1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman heart tissue, human brain tissue, human placenta tissue, human liver tissue, mouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

12630-1-AP targets PLSCR4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PLSCR4 fusion protein Ag3323 相同性解析による交差性が予測される生物種
Full Name phospholipid scramblase 4
Calculated molecular weight 329 aa, 37 kDa
Observed molecular weight 37 kDa
GenBank accession numberBC028354
Gene Symbol PLSCR4
Gene ID (NCBI) 57088
RRIDAB_2165948
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NRQ2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PLSCR4 (Phospholipid scramblase 4) belongs to the phospholipid scramblase family which is thought to mediate the loss of lipid asymmetry. Elimination of this lipid bilayer asymmetry is an important physiological process and appears to occur as a result of elevated intracellular calcium concentration, or occur with cell activation, injury, and apoptosis.PLSCR4 also plays a central role in the initiation of fibrin clot formation, in the activation of mast cells and in the recognition of apoptotic and injured cells by the reticuloendothelial system.

Protocols

Product Specific Protocols
WB protocol for PLSCR4 antibody 12630-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Reprod Sci

Phospholipid scramblase expression in the pregnant mouse uterus in LPS-induced preterm delivery.

Authors - McLean Kelley C KC
View Article