Validation Data Gallery
Tested Applications
| Positive WB detected in | human heart tissue, human brain tissue, human placenta tissue, human liver tissue, mouse testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
12630-1-AP targets PLSCR4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3323 Product name: Recombinant human PLSCR4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-272 aa of BC028354 Sequence: MSGVVPTAPEQPAGEMENQTKPPDPRPDAPPEYNSHFLPGPPGTAVPPPTGYPGGLPMGYYSPQQPSTFPLYQPVGGIHPVRYQPGKYPMPNQSVPITWMPGPTPMANCPPGLEYLVQLDNIHVLQHFEPLEMMTCFETNNRYDIKNNSDQMVYIVTEDTDDFTRNAYRTLRPFVLRVTDCMGREIMTMQRPFRCTCCCFCCPSARQELEVQCPPGVTIGFVAEHWNLCRAVYSIQNEKKENVMRVRGPCSTYGCGSDSVFEVKSLDGISNI 相同性解析による交差性が予測される生物種 |
| Full Name | phospholipid scramblase 4 |
| Calculated molecular weight | 329 aa, 37 kDa |
| Observed molecular weight | 37 kDa |
| GenBank accession number | BC028354 |
| Gene Symbol | PLSCR4 |
| Gene ID (NCBI) | 57088 |
| RRID | AB_2165948 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NRQ2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PLSCR4 (Phospholipid scramblase 4) belongs to the phospholipid scramblase family which is thought to mediate the loss of lipid asymmetry. Elimination of this lipid bilayer asymmetry is an important physiological process and appears to occur as a result of elevated intracellular calcium concentration, or occur with cell activation, injury, and apoptosis.PLSCR4 also plays a central role in the initiation of fibrin clot formation, in the activation of mast cells and in the recognition of apoptotic and injured cells by the reticuloendothelial system.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PLSCR4 antibody 12630-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |