Validation Data Gallery

Filter: AllWBIPIHCIF-FroIF/ICC

WB analysis using 11582-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11582-1-AP (PLSCR1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 11582-1-AP
WB result of PLSCR1 antibody (11582-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PLSCR1 transfected HEK-293 cells.
WB analysis using 11582-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11582-1-AP (PLSCR1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis using 11582-1-AP
Mouse lung lysates were subjected to SDS PAGE followed by western blot with 11582-1-AP (PLSCR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 11582-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11582-1-AP (PLSCR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human skeletal muscle using 11582-1-AP
human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11582-1-AP (PLSCR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IP experiment of mouse lung using 11582-1-AP
IP result of anti-PLSCR1 (IP:11582-1-AP, 4ug; Detection:11582-1-AP 1:500) with mouse lung tissue lysate 2800ug.
IHC staining of mouse spleen using 11582-1-AP
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 11582-1-AP (PLSCR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 11582-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11582-1-AP (PLSCR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 11582-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 11582-1-AP (PLSCR1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 11582-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 11582-1-AP (PLSCR1 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of mouse spleen using 11582-1-AP
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 11582-1-AP (PLSCR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse brain using 11582-1-AP
Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using PLSCR1 antibody (11582-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
IF Staining of rat brain using 11582-1-AP
Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded rat brain tissue using PLSCR1 antibody (11582-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
IF Staining of HeLa using 11582-1-AP
Immunofluorescent analysis of HeLa cells using 11582-1-AP (PLSCR1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

PLSCR1 Polyclonal antibody

PLSCR1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-Fro, IP, ELISA

Cat No. 11582-1-AP

Publications(18)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-Fro, IP, ELISA

Synonyms

Ca(2+)-dependent phospholipid scramblase 1, EC:3.1.-.-, Mg(2+)-dependent nuclease, MMTRA1B, phospholipid scramblase 1

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (18)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, human skeletal muscle tissue, mouse skeletal muscle tissue, mouse lung tissue, A549 cells, HepG2 cells, human placenta tissue
Positive IP detected in	mouse lung tissue
Positive IHC detected in	mouse spleen tissue, human kidney tissue, human pancreas cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-Fro detected in	rat brain tissue, mouse brain tissue
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-FRO	IF-FRO : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 8 publications below
WB	See 14 publications below
IHC	See 5 publications below
IF	See 8 publications below
IP	See 2 publications below

Product Information

11582-1-AP targets PLSCR1 in WB, IHC, IF/ICC, IF-Fro, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PLSCR1 fusion protein Ag2172 相同性解析による交差性が予測される生物種
Full Name	phospholipid scramblase 1
Calculated molecular weight	318 aa, 35 kDa
Observed molecular weight	35 kDa
GenBank accession number	BC021100
Gene Symbol	PLSCR1
Gene ID (NCBI)	5359
RRID	AB_2165659
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O15162
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PLSCR1 (Phospholipid scramblase 1) is a member of the family of membrane proteins that mediate the transbilayer movement of phospholipids ("scrambling") in a Ca2+ dependent manner, which was identified as an a type II transmembrane protein involved in the calcium-dependent, nonspecific, rapid redistribution of phospholipids. A cysteine-rich palmitoylation motif [184CCCPCC189] of PLSCR1, a thioester linkage to the sulfhydryl groups of cysteines, controls the transportation of PLSCR1 to the cell membrane or nucleus (PMID: 32292520). PLSCR1 is strongly expressed in response to interferon (IFN) treatment and viral infection (PMID: 35138119, 23487022).

Protocols

Product Specific Protocols
WB protocol for PLSCR1 antibody 11582-1-AP	Download protocol
IHC protocol for PLSCR1 antibody 11582-1-AP	Download protocol
IF protocol for PLSCR1 antibody 11582-1-AP	Download protocol
IP protocol for PLSCR1 antibody 11582-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB,IF	J Clin Invest Phospholipid scramblase-1 regulates innate type 2 inflammation in mouse lung via CRTH2-dependent mechanisms Authors - Ashley Hernandez-Gutierrez View Article
human	WB	PLoS Pathog Phospholipid scramblase 1 interacts with influenza A virus NP, impairing its nuclear import and thereby suppressing virus replication. Authors - Weiyu Luo View Article
mouse	WB	J Neurosci Phospholipid Scramblase 1 Controls Efficient Neurotransmission and Synaptic Vesicle Retrieval at Cerebellar Synapses Authors - Margherita Caputo KD Validated View Article
human	WB,IF	J Proteome Res An Interactome Map Reveals Phospholipid Scramblase 1 as a Novel Regulator of Hepatitis B Virus X Protein. Authors - Yanzhi Yuan KD Validated View Article
human	WB,IP,IF	Cancer Sci Nuclear accumulation of KPNA2 impacts radioresistance through positive regulation of the PLSCR1-STAT1 loop in lung adenocarcinoma. Authors - Wei-Chao Liao KD Validated View Article
mouse	WB	J Leukoc Biol Phospholipid scramblase 1 is required for β2-glycoprotein I binding in hypoxia and reoxygenation-induced endothelial inflammation. Authors - Emily Archer Slone View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 11582-1-AP

WB analysis of HEK-293 using 11582-1-AP

WB analysis using 11582-1-AP

WB analysis using 11582-1-AP

WB analysis of mouse skeletal muscle using 11582-1-AP

WB analysis of human skeletal muscle using 11582-1-AP

IP experiment of mouse lung using 11582-1-AP

IHC staining of mouse spleen using 11582-1-AP

IHC staining of human pancreas cancer using 11582-1-AP

IHC staining of human kidney using 11582-1-AP

IHC staining of human kidney using 11582-1-AP

IHC staining of mouse spleen using 11582-1-AP

IF Staining of mouse brain using 11582-1-AP

IF Staining of rat brain using 11582-1-AP

IF Staining of HeLa using 11582-1-AP

PLSCR1 Polyclonal antibody

PLSCR1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-Fro, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Phospholipid scramblase-1 regulates innate type 2 inflammation in mouse lung via CRTH2-dependent mechanisms

Phospholipid scramblase 1 interacts with influenza A virus NP, impairing its nuclear import and thereby suppressing virus replication.

Phospholipid Scramblase 1 Controls Efficient Neurotransmission and Synaptic Vesicle Retrieval at Cerebellar Synapses

An Interactome Map Reveals Phospholipid Scramblase 1 as a Novel Regulator of Hepatitis B Virus X Protein.

Nuclear accumulation of KPNA2 impacts radioresistance through positive regulation of the PLSCR1-STAT1 loop in lung adenocarcinoma.

Phospholipid scramblase 1 is required for β2-glycoprotein I binding in hypoxia and reoxygenation-induced endothelial inflammation.