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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse small intestine tissue using PLS1 Polyclonal antibody (55212-1-AP)

    WB analysis of mouse small intestine using 55212-1-AP (same clone as 55212-1-PBS)

    mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 55212-1-AP (PLS1 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55212-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse small intestine tissue using PLS1 Polyclonal antibody (55212-1-AP)

    WB analysis of mouse small intestine using 55212-1-AP (same clone as 55212-1-PBS)

    mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 55212-1-AP (PLS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55212-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using PLS1 Polyclonal antibody (55212-1-AP)

    WB analysis of COLO 320 using 55212-1-AP (same clone as 55212-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 55212-1-AP (PLS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55212-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using PLS1 Polyclonal antibody (55212-1-AP)

    WB analysis of COLO 320 using 55212-1-AP (same clone as 55212-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 55212-1-AP (PLS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55212-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse small intestine tissue using PLS1 Polyclonal antibody (55212-1-AP)

    WB analysis of mouse small intestine using 55212-1-AP (same clone as 55212-1-PBS)

    mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 55212-1-AP (PLS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55212-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using PLS1 Polyclonal antibody (55212-1-AP)

    WB analysis of COLO 320 using 55212-1-AP (same clone as 55212-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 55212-1-AP (PLS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55212-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using PLS1 Polyclonal antibody (55212-1-AP)

    WB analysis of COLO 320 using 55212-1-AP (same clone as 55212-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 55212-1-AP (PLS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55212-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PLS1 Polyclonal antibody (55212-1-AP)

    IHC staining of human colon cancer using 55212-1-AP (same clone as 55212-1-PBS)

    Immunohistochemical analysis of paraffin-embedded colon cancer slide using 55212-1-AP (PLS1 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55212-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using PLS1 Polyclonal antibody (55212-1-AP)

    IHC staining of human kidney using 55212-1-AP (same clone as 55212-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 55212-1-AP (PLS1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 55212-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using PLS1 Polyclonal antibody (55212-1-AP)

    IHC staining of human kidney using 55212-1-AP (same clone as 55212-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 55212-1-AP (PLS1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55212-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

PLS1 Polyclonal antibody, PBS Only

PLS1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 55212-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Plastin-1, Plastin 1, I-plastin, Intestine-specific plastin, I plastin

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

55212-1-PBS targets PLS1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name plastin 1 (I isoform)
Calculated molecular weight 70 kDa
Observed molecular weight 70 kDa
GenBank accession numberNM_002670
Gene Symbol PLS1
Gene ID (NCBI) 5357
RRIDAB_10859080
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14651
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PLS1, also named as I-plastin and Plastin-1, is an actin-bundling protein in the absence of calcium. It is intestine-specific plastin. This antibody is specific to PLS1. This antibody is specific to PLS1. It has no cross reaction to PLS3.