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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

PLP2 Polyclonal antibody

PLP2 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21482-1-AP

Synonyms

A4, A4 LSB, Intestinal membrane A4 protein, PLP2, Proteolipid protein 2

Validation Data Gallery

Applications:
  • WB analysis using 21482-1-AP

    WB analysis using 21482-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21482-1-AP (PLP2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 21482-1-AP

    IHC staining of human colon using 21482-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 21482-1-AP (PLP2 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with PLP2 antibody included
  • IHC staining of human colon using 21482-1-AP

    IHC staining of human colon using 21482-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 21482-1-AP (PLP2 antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with PLP2 antibody included
  • IF Staining of HepG2 using 21482-1-AP

    IF Staining of HepG2 using 21482-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 21482-1-AP (PLP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inSMMC-7721 cells, HEK-293 cells, COLO 320 cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy PLP2 Ready-To-Use IHC Kit. PLP2 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21482-1-AP is PLP2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PLP2 fusion protein Ag15740 相同性解析による交差性が予測される生物種
Full Name proteolipid protein 2 (colonic epithelium-enriched)
Calculated molecular weight 152 aa, 17 kDa
Observed molecular weight 15-17 kDa
GenBank accession numberBC109066
Gene symbol PLP2
Gene ID (NCBI) 5355
RRIDAB_10858923
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PLP2 antibody 21482-1-APDownload protocol
IHC protocol for PLP2 antibody 21482-1-APDownload protocol
IF protocol for PLP2 antibody 21482-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Cell Physiol

The long noncoding RNA ZFAS1 promotes the progression of glioma by regulating the miR-150-5p/PLP2 axis.

Authors - Xiaojian Li
  • KD Validated
View Article
humanIHC

Biomed Res Int

PLP2 Expression as a Prognostic and Therapeutic Indicator in High-Risk Multiple Myeloma.

Authors - Hua Bai
View Article