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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PLOD3 Monoclonal antibody (60058-1-Ig)

    WB analysis using 60058-1-Ig (same clone as 60058-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60058-1-Ig (PLOD3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 60058-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using PLOD3 Monoclonal antibody (60058-1-Ig)

    WB analysis of human placenta using 60058-1-Ig (same clone as 60058-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 60058-1-Ig (PLOD3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60058-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using PLOD3 Monoclonal antibody (60058-1-Ig)

    WB analysis of HepG2 using 60058-1-Ig (same clone as 60058-1-PBS)

    WB result of PLOD3 antibody (60058-1-Ig, 1:2000) with si-Control and si-PLOD3 transfected HepG2 cells. This data was developed using the same antibody clone with 60058-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PLOD3 Monoclonal antibody (60058-1-Ig)

    WB analysis using 60058-1-Ig (same clone as 60058-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60058-1-Ig (PLOD3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60058-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using PLOD3 Monoclonal antibody (60058-1-Ig)

    WB analysis of PC-3 using 60058-1-Ig (same clone as 60058-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 60058-1-Ig (PLOD3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60058-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using PLOD3 Monoclonal antibody (60058-1-Ig)

    IP experiment of HeLa using 60058-1-Ig (same clone as 60058-1-PBS)

    IP result of anti-PLOD3 (IP:60058-1-Ig, 5ug; Detection:60058-1-Ig 1:500) with HeLa cells lysate 1400ug. This data was developed using the same antibody clone with 60058-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using PLOD3 Monoclonal antibody (60058-1-Ig)

    IHC staining of human pancreas cancer using 60058-1-Ig (same clone as 60058-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 60058-1-Ig(PLOD3 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 60058-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using PLOD3 Monoclonal antibody (60058-1-Ig)

    IHC staining of human pancreas cancer using 60058-1-Ig (same clone as 60058-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 60058-1-Ig(PLOD3 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 60058-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PLOD3 Monoclonal antibody (60058-1-Ig)

    IF Staining of HeLa using 60058-1-Ig (same clone as 60058-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PLOD3 antibody (60058-1-Ig, Clone: 2G12B8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60058-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PLOD3 Monoclonal antibody (60058-1-Ig)

    IF Staining of HeLa using 60058-1-Ig (same clone as 60058-1-PBS)

    Immunofluorescent analysis of HeLa cells using 60058-1-Ig (PLOD3 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG. This data was developed using the same antibody clone with 60058-1-PBS in a different storage buffer formulation.

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  • KD/KO Validated

PLOD3 Monoclonal antibody, PBS Only

PLOD3 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 60058-1-PBS
Clone No.2G12B8

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Procollagen galactosyltransferase, Multifunctional procollagen lysine hydroxylase and glycosyltransferase LH3, Lysyl hydroxylase 3, LH3, Galactosylhydroxylysine-glucosyltransferase

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60058-1-PBS targets PLOD3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen PLOD3 fusion protein Ag1480 相同性解析による交差性が予測される生物種
Full Name procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3
Calculated molecular weight 738 aa, 85 kDa
Observed molecular weight 80-85 kDa
GenBank accession numberBC011674
Gene Symbol PLOD3
Gene ID (NCBI) 8985
RRIDAB_2165779
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO60568
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

PLOD3, also named as LH3, forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links. The major function of PLOD3 in osteoblasts is to glucosylate galactosylhydroxylysine residues in type I collagen.