Validation Data Gallery
Tested Applications
| Positive WB detected in | DU 145 cells, HSC-T6 cells, NIH/3T3 cells, A549 cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
88222-2-RR targets PLOD2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag15614 Product name: Recombinant human PLOD2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 459-540 aa of BC037169 Sequence: PYMANVYLIKGKTLRSEMNERNYFVRDKLDPDMALCRNAREMTLQREKDSPTPETFQMLSPPKGVFMYISNRHEFGRLLSTA 相同性解析による交差性が予測される生物種 |
| Full Name | procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2 |
| Calculated molecular weight | 758 aa, 85 kDa |
| Observed molecular weight | 85 kDa |
| GenBank accession number | BC037169 |
| Gene Symbol | PLOD2 |
| Gene ID (NCBI) | 5352 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O00469 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PLOD2,also named as LH2, forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. It is a potential novel prognostic factor for HCC patients following surgery. Among the PLOD genes, PLOD2 contributes to cancer prognosis and angiogenesis. Several authors have reported that PLOD2 expression might provide prognostic information about malignant tumours such as glioblastoma. PLOD2 expression is a useful biomarker for the effects of antiangiogenic treatment for malignancy. (PMID:22098155). It has 2 isoforms produced by alternative splicing and seven glycosylation sites.This antibody is specific to PLOD2.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PLOD2 antibody 88222-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |