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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PLK4 Polyclonal antibody (28750-1-AP)

    IHC staining of human colon cancer using 28750-1-AP (same clone as 28750-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28750-1-AP (PLK4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28750-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PLK4 Polyclonal antibody (28750-1-AP)

    IHC staining of human colon cancer using 28750-1-AP (same clone as 28750-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28750-1-AP (PLK4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28750-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using PLK4 Polyclonal antibody (28750-1-AP)

    IHC staining of mouse testis using 28750-1-AP (same clone as 28750-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28750-1-AP (PLK4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28750-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using PLK4 Polyclonal antibody (28750-1-AP)

    IHC staining of mouse testis using 28750-1-AP (same clone as 28750-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28750-1-AP (PLK4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28750-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using PLK4 Polyclonal antibody (28750-1-AP)

    IF Staining of hTERT-RPE1 using 28750-1-AP (same clone as 28750-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using PLK4 antibody (28750-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 ARL13B antibody (CL594-17711, red). This data was developed using the same antibody clone with 28750-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

PLK4 Polyclonal antibody, PBS Only

PLK4 Polyclonal Antibody for IHC, IF/ICC, Indirect ELISA
Cat No. 28750-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

28750-1-PBS targets PLK4 in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag30482

Product name: Recombinant human PLK4 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 235-426 aa of BC036023

Sequence: SIEAKDLIHQLLRRNPADRLSLSSVLDHPFMSRNSSTKSKDLGTVEDSIDSGHATISTAITASSSTSISGSLFDKRRLLIGQPLPNKMTVFPKNKSSTDFSSSGDGNSFYTQWGNQETSNSGRGRVIQDAEERPHSRYLRRAYSSDRSGTSNSQSQAKTYTMERCHSAEMLSVSKRSGGGENEERYSPTDNN

相同性解析による交差性が予測される生物種
Full Name polo-like kinase 4 (Drosophila)
Calculated molecular weight 970 aa, 109 kDa
GenBank accession numberBC036023
Gene Symbol PLK4
Gene ID (NCBI) 10733
RRIDAB_2881206
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO00444
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PLK4 (Polo-like kinase 4) is also named SAK, STK18 and belongs to the Ser/Thr protein kinase family. It plays a central role in centriole duplication and is a kinase implicated in tumor development, which is an upstream regulator of canonical biogenesis necessary for centriole formation. It has 3 isoforms produced by alternative splicing.

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