Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells, K-562 cells |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 6 publications below |
| IHC | See 1 publications below |
Product Information
24883-1-AP targets PLEKHO1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | rat, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag19309 Product name: Recombinant human PLEKHO1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 176-409 aa of BC010149 Sequence: ASTSTSDGMLTLDLIQEEDPSPEEPTSCAESFRVDLDKSVAQLAGSRRRADSDRIQPSADRASSLSRPWEKTDKGATYTPQAPKKLTPTEKGRCASLEEILSQRDAASARTLQLRAEEPPTPALPNPGQLSRIQDLVARKLEETQELLAEVQGLGDGKRKAKDPPRSPPDSESEQLLLETERLLGEASSNWSQAKRVLQEVRELRDLYRQMDLQTPDSHLRQTTPHSQYRKSLM 相同性解析による交差性が予測される生物種 |
| Full Name | pleckstrin homology domain containing, family O member 1 |
| Calculated molecular weight | 409 aa, 46 kDa |
| Observed molecular weight | 46 kDa, 90 kDa |
| GenBank accession number | BC010149 |
| Gene Symbol | PLEKHO1 |
| Gene ID (NCBI) | 51177 |
| RRID | AB_2879778 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q53GL0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PLEKHO1, also named as CK2 interacting protein 1 or OC120, is a 409 amino acid protein, which contains one PH domain. PLEKHO1 Predominantly localizes to the plasma membrane. PLEKHO1 plays a role in the regulation of the actin cytoskeleton through its interactions with actin capping protein (CP). Catalog#24883-1-AP recognizes 46 kDa and 90 kDa homodimer band.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PLEKHO1 antibody 24883-1-AP | Download protocol |
| IHC protocol for PLEKHO1 antibody 24883-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Bioact Mater Genetically engineered M2-like macrophage-derived exosomes for P. gingivalis-suppressed cementum regeneration: From mechanism to therapy | ||
J Dent Res Ckip-1 Mediates P. gingivalis-Suppressed Cementoblast Mineralization.
| ||
Front Physiol Myocardial CKIP-1 Overexpression Protects from Simulated Microgravity-Induced Cardiac Remodeling. | ||
Front Physiol Casein Kinase-2 Interacting Protein-1 Regulates Physiological Cardiac Hypertrophy via Inhibition of Histone Deacetylase 4 Phosphorylation. | ||
Front Pharmacol Exploratory study of sea buckthorn enhancing QiangGuYin efficacy by inhibiting CKIP-1 and Notum activating the Wnt/β-catenin signaling pathway and analysis of active ingredients by molecular docking | ||
J Oral Microbiol CKIP-1 mediates P. gingivalis-suppressed osteogenic/cementogenic differentiation of periodontal ligament cells partially via p38 signaling pathway |