Validation Data Gallery
Tested Applications
| Positive WB detected in | A431 cells, HT-29 cells, mouse brain tissue, mouse cerebellum tissue, rat brain tissue, rat cerebellum tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
33028-1-AP targets PLEKHN1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag38741 Product name: Recombinant human PLEKHN1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 200-350 aa of BC101386 Sequence: SAPPQGSCGDELPWTLQRRLTRLRTASGHEPGGSAVCASRVKLQHLPAQEQWDRLLVLYPTSLAIFSEELDGLCFKGELPLRAVHINLEEKEKQIRSFLIEGPLINTIRVVCASYEDYGHWLLCLRAVTHREGAPPLPGAESFPGSQVMGS 相同性解析による交差性が予測される生物種 |
| Full Name | pleckstrin homology domain containing, family N member 1 |
| Calculated molecular weight | 663 aa, 72 kDa |
| Observed molecular weight | 66-71 kDa |
| GenBank accession number | BC101386 |
| Gene Symbol | PLEKHN1 |
| Gene ID (NCBI) | 84069 |
| RRID | AB_3742825 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q494U1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PLEKHN1 (Pleckstrin Homology Domain Containing N1) is a protein-coding gene located on chromosome 1. It plays a crucial role in various cellular processes, including apoptosis and mRNA stability regulation. PLEKHN1 has been identified as a pro-apoptotic protein that enhances Bax-Bak hetero-oligomerization via interaction with Bid in colon cancer cells. (PMID: 29531808)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PLEKHN1 antibody 33028-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |