Validation Data Gallery

Filter: AllWBIHC

WB analysis of ROS1728 using 16202-1-AP
ROS1728 cells were subjected to SDS PAGE followed by western blot with 16202-1-AP (PLEKHM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 16202-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 16202-1-AP (PLEKHM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of human kidney using 16202-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16202-1-AP (PLEKHM1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 16202-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16202-1-AP (PLEKHM1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 16202-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16202-1-AP (PLEKHM1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 16202-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16202-1-AP (PLEKHM1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 16202-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16202-1-AP (PLEKHM1 antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human liver cancer using 16202-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16202-1-AP (PLEKHM1 antibody) at dilution of 1:200 (under 40x lens).

PLEKHM1 Polyclonal antibody

PLEKHM1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 16202-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

AP162, OPTB6, PH domain-containing family M member 1, Pleckstrin homology domain-containing family M member 1

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (3)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	ROS1728 cells, mouse liver tissue
Positive IHC detected in	human kidney tissue, human liver cancer tissue, human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:200-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 2 publications below

Product Information

16202-1-AP targets PLEKHM1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PLEKHM1 fusion protein Ag9145 相同性解析による交差性が予測される生物種
Full Name	pleckstrin homology domain containing, family M (with RUN domain) member 1
Calculated molecular weight	117 kDa
Observed molecular weight	117 kDa
GenBank accession number	BC064361
Gene Symbol	PLEKHM1
Gene ID (NCBI)	9842
RRID	AB_2878228
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9Y4G2
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PLEKHM1, also known as AP162, OPTB6, belongs to the Pleckstrin homology domain protein family. PLEKHM1 functions as a multivalent adapter protein that regulates Rab7-dependent and HOPS complex-dependent fusion events within the endolysosomal system, thereby coupling autophagic and endocytic trafficking pathways. In osteoclasts, PLEKHM1 is crucial for bone resorption by regulating the vesicle transport process and ensuring the formation of the ruffled border.

Protocols

Product Specific Protocols
WB protocol for PLEKHM1 antibody 16202-1-AP	Download protocol
IHC protocol for PLEKHM1 antibody 16202-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Autophagy SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes Authors - Wang Zheng View Article
mouse	WB	Nutrients Improving Lipophagy by Restoring Rab7 Cycle: Protective Effects of Quercetin on Ethanol-Induced Liver Steatosis. Authors - Hongkun Lin View Article
		Cell Biol Toxicol Downregulation of MYBL1 in endothelial cells contributes to atherosclerosis by repressing PLEKHM1-inducing autophagy Authors - Shi-Ao Ding View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of ROS1728 using 16202-1-AP

WB analysis of mouse liver using 16202-1-AP

IHC staining of human kidney using 16202-1-AP