Validation Data Gallery
Tested Applications
| Positive WB detected in | Caco-2 cells, MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21677-1-AP targets PLEKHG6 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag15886 Product name: Recombinant human PLEKHG6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 456-790 aa of BC105961 Sequence: LMLEKLVCQPLRDPNSFLLIHLTEFQCVSSALLVHCPSPTDRAQWLEKTQQAQAALQKLKAEEYVQQKRELLTLYRDQDRESPSTRPSTPSLEGSQSSAEGRTPEFSTIIPHLVVTEDTDEDAPLVPDDTSDSGYGTLIPGTPTGSRSPLSRLRQRALRRDPRLTFSTLELRDIPLRPHPPDPQAPQRRSAPELPEGILKGGSLPQEDPPTWSEEEDGASERGNVVVETLHRARLRGQLPSSPTHADSAGESPWESSGEEEEEGPLFLKAGHTSLRPMRAEDMLREIREELASQRIEGAEEPRDSRPRKLTRAQLQRMRGPHIIQLDTPLSASEV 相同性解析による交差性が予測される生物種 |
| Full Name | pleckstrin homology domain containing, family G (with RhoGef domain) member 6 |
| Calculated molecular weight | 790 aa, 89 kDa |
| Observed molecular weight | 89 kDa |
| GenBank accession number | BC105961 |
| Gene Symbol | PLEKHG6 |
| Gene ID (NCBI) | 55200 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q3KR16 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PLEKHG6 is a previously unknown guanine nucleotide exchange factor (GEF) for RhoG. It localizes to the apical membrane of cells, a process that depends on its interaction with the membrane-cytoskeleton linker protein ezrin. This apical localization is essential for inducing morphological changes. Additionally, PLEKHG6 and ezrin are both necessary for macropinocytosis mediated by membrane ruffles. Finally, ezrin facilitates precise spatial regulation of RhoG activation at the apical surface of epithelial cells by recruiting components of the RhoG signaling pathway into a closely associated complex.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PLEKHG6 antibody 21677-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |