Validation Data Gallery
Tested Applications
| Positive WB detected in | HUVEC cells, HeLa cells, SH-SY5Y cells, SH-N-SH cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
17156-1-AP targets PLEKHG4 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10328 Product name: Recombinant human PLEKHG4 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-350 aa of BC082974 Sequence: MHVKDPGPPRPPAGATQDEELQGSPLSRKFQLPPAADESGDAQRGTVESSSVLSEGPGPSGVESLLCPMSSHLSLAQGESDTPGVGLVGDPGPSRAMPSGLSPGALDSDPVGLGDPLSEISKLLEAAPSGSGLPKPADCLLAQDLCWELLASGMATLPGTRDVQGRAVLLLCAHSPAWLQSECSSQELIRLLLYLRSIPRPEVQALGLTVLVDARICAPSSSLFSGLSQLQEAAPGAVYQVLLVGSTLLKEVPSGLQLEQLPSQSLLTHIPTAGLPTSLGGGLPYCHQAWLDFRRRLEALLQNCQAACALLQGAIESVKAVPQPMEPGEVGQLLQQTEVLMQQVLDSPWL 相同性解析による交差性が予測される生物種 |
| Full Name | pleckstrin homology domain containing, family G (with RhoGef domain) member 4 |
| Calculated molecular weight | 1151 aa, 126 kDa |
| Observed molecular weight | 131 kDa |
| GenBank accession number | BC082974 |
| Gene Symbol | PLEKHG4 |
| Gene ID (NCBI) | 25894 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q58EX7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The PLEKHG4 protein (full name: Pleckstrin Homology and RhoGEF Domain Containing G4), also known as Puratrophin-1, belongs to the Rho guanine nucleotide exchange factor (RhoGEF) family containing Dbl homology (DH) domains and pleckstrin homology (PH) domains. Its core function is to act as a GEF, catalyzing the conversion of small GTPases of the Rho family from the inactive GDP-bound state to the active GTP-bound state, thereby playing a key role in intracellular signal transduction and Golgi cytoskeletal dynamics.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PLEKHG4 antibody 17156-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |