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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PLEKHF2 Polyclonal antibody (25424-1-AP)

    WB analysis using 25424-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25424-1-AP (PLEKHF2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brown adipose tissue using PLEKHF2 Polyclonal antibody (25424-1-AP)

    IHC staining of mouse brown adipose using 25424-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 25424-1-AP (PLEKHF2 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat brown adipose tissue using PLEKHF2 Polyclonal antibody (25424-1-AP)

    IHC staining of rat brown adipose using 25424-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brown adipose tissue slide using 25424-1-AP (PLEKHF2 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat brown adipose tissue using PLEKHF2 Polyclonal antibody (25424-1-AP)

    IHC staining of rat brown adipose using 25424-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brown adipose tissue slide using 25424-1-AP (PLEKHF2 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse brown adipose tissue using PLEKHF2 Polyclonal antibody (25424-1-AP)

    IF Staining of mouse brown adipose using 25424-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brown adipose tissue using PLEKHF2 antibody (25424-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse brown adipose tissue using PLEKHF2 Polyclonal antibody (25424-1-AP)

    IF Staining of mouse brown adipose using 25424-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brown adipose tissue using PLEKHF2 antibody (25424-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PLEKHF2 Polyclonal antibody

PLEKHF2 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 25424-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

Phafin 2, PHAFIN2, ZFYVE18

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inrat brown adipose tissue, mouse brown adipose tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brown adipose tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25424-1-AP targets PLEKHF2 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag22285

Product name: Recombinant human PLEKHF2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 186-249 aa of BC011806

Sequence: CSEKRFLLPSQSSKPVRICDFCYDLLSAGDMATCQPARSDSYSQSLKSPLNDMSDDDDDDDSSD

相同性解析による交差性が予測される生物種
Full Name pleckstrin homology domain containing, family F (with FYVE domain) member 2
Calculated molecular weight 249 aa, 28 kDa
Observed molecular weight22-27 kDa
GenBank accession numberBC011806
Gene Symbol PLEKHF2
Gene ID (NCBI) 79666
RRIDAB_3669474
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H8W4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Phafin2 is a lysosomal protein consisting of 249 amino acids with a unique structure containing both an N-terminal PH (pleckstrin homology) domain and C terminal FYVE (Fab 1, YOTB, Vac 1, and EEA1) domain.

Protocols

Product Specific Protocols
IF protocol for PLEKHF2 antibody 25424-1-APDownload protocol
IHC protocol for PLEKHF2 antibody 25424-1-APDownload protocol
WB protocol for PLEKHF2 antibody 25424-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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