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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PLEK2 Recombinant antibody (85619-4-RR)

    WB analysis using 85619-4-RR

    HT-29, U2OS, PC-3, Caco-2 (positive) and Ramos (negative) cells were subjected to SDS PAGE followed by western blot with 85619-4-RR (PLEK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 85619-4-RR

    Affinity and Kinetic Characterization of 85619-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85619-4-RR against Human PLEK2 were performed. The affinity constant is below 1 pM.

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PLEK2 Recombinant antibody

PLEK2 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 85619-4-RR
Clone No.242987B7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Pleckstrin 2, Pleckstrin-2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHT-29 cells, U2OS cells, PC-3 cells, Caco-2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85619-4-RR targets PLEK2 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PLEK2 fusion protein Ag2297 相同性解析による交差性が予測される生物種
Full Name pleckstrin 2
Calculated molecular weight 353 aa, 40 kDa
Observed molecular weight 38-40 kDa
GenBank accession numberBC001226
Gene Symbol PLEK2
Gene ID (NCBI) 26499
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NYT0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PLEK2 belongs to the Pleckstrin homology domain family and is a 40-kDa protein containing the prototypic PH domains at its amino and carboxyl termini(PMID: 10419454). PLEK2 associates with membrane-bound phosphatidylinositols generated by phosphatidylinositol 3-kinase. PLEK2 interacts with the actin cytoskeleton to induce cell spreading.

Protocols

Product Specific Protocols
WB protocol for PLEK2 antibody 85619-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols