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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PLDN Polyclonal antibody (10891-2-AP)

    WB analysis using 10891-2-AP (same clone as 10891-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10891-2-AP (PLDN antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10891-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using PLDN Polyclonal antibody (10891-2-AP)

    WB analysis of A431 using 10891-2-AP (same clone as 10891-2-PBS)

    WB result of PLDN antibody (10891-2-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PLDN transfected A431 cells. This data was developed using the same antibody clone with 10891-2-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of K-562 cells using PLDN Polyclonal antibody (10891-2-AP)

    IP experiment of K-562 using 10891-2-AP (same clone as 10891-2-PBS)

    IP result of anti-PLDN (IP:10891-2-AP, 3ug; Detection:10891-2-AP 1:500) with K-562 cells lysate 3600ug. This data was developed using the same antibody clone with 10891-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PLDN Polyclonal antibody (10891-2-AP)

    IHC staining of human breast cancer using 10891-2-AP (same clone as 10891-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10891-2-AP (PLDN antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10891-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PLDN Polyclonal antibody (10891-2-AP)

    IHC staining of human breast cancer using 10891-2-AP (same clone as 10891-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10891-2-AP (PLDN antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10891-2-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

PLDN Polyclonal antibody, PBS Only

PLDN Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 10891-2-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10891-2-PBS targets PLDN in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1337

Product name: Recombinant human PLDN protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-172 aa of BC004819

Sequence: MSVPGPSSPDGALTRPPYCLEAGEPTPGLSDTSPDEGLIEDLTIEDKAVEQLAEGLLSHYLPDLQRSKQALQELTQNQVVLLDTLEQEISKFKECHSMLDINALFAEAKHYHAKLVNIRKEMLMLHEKTSKLKKRALKLQQKRQKEELEREQQREKEFEREKQLTARPAKRM

相同性解析による交差性が予測される生物種
Full Name pallidin homolog (mouse)
Calculated molecular weight 23~24 kDa
Observed molecular weight 25 kDa
GenBank accession numberBC004819
Gene Symbol PLDN
Gene ID (NCBI) 26258
RRIDAB_2164174
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UL45
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Pallidin, also named as PLDN and PA, is involved in the development of lysosome-related organelles, such as melanosomes and platelet-dense granules. It may play a role in intracellular vesicle trafficking, particularly in the vesicle-docking and fusion process. Pallidin interacts with syntaxin-12 which is a t-SNARE protein that mediates vesicle docking and fusion. Pallidin is a 25 kDa subunit of the octamer BLOC-1.

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