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  • Featured Product
  • KD/KO Validated

PLD3 Polyclonal antibody

PLD3 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 17327-1-AP

Synonyms

Choline phosphatase 3, HindIII K4L homolog, HU K4, Phospholipase D3, PLD 3, PLD3

Validation Data Gallery

Applications:
  • WB analysis using 17327-1-AP

    WB analysis using 17327-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17327-1-AP (PLD3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis using 17327-1-AP

    WB analysis using 17327-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17327-1-AP (PLD3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis using 17327-1-AP

    WB analysis using 17327-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17327-1-AP (PLD3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis using 17327-1-AP

    WB analysis using 17327-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17327-1-AP (PLD3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse brain using 17327-1-AP

    IF Staining of mouse brain using 17327-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PLD3 antibody (17327-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of A431 using 17327-1-AP

    IF Staining of A431 using 17327-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using PLD3 antibody (17327-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse brain using 17327-1-AP

    IF Staining of mouse brain using 17327-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PLD3 antibody (17327-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, SH-SY5Y cells
Positive IF detected inmouse brain tissue, A431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

17327-1-AP targets PLD3 in WB, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PLD3 fusion protein Ag11306 相同性解析による交差性が予測される生物種
Full Name phospholipase D family, member 3
Calculated molecular weight 490 aa, 55 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC036327
Gene symbol PLD3
Gene ID (NCBI) 23646
RRIDAB_2878383
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PLD3 antibody 17327-1-APDownload protocol
IF protocol for PLD3 antibody 17327-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Hum Mol Genet

Pathological characterization of a novel mouse model expressing the PD-linked CHCHD2-T61I mutation.

Authors - Teresa R Kee
View Article
humanFC

Cell Death Discov

A signature of enhanced lipid metabolism, lipid peroxidation and aldehyde stress in therapy-induced senescence.

Authors - Amy C Flor
View Article