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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PLD3 Polyclonal antibody (17327-1-AP)

    WB analysis using 17327-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17327-1-AP (PLD3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PLD3 Polyclonal antibody (17327-1-AP)

    WB analysis using 17327-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17327-1-AP (PLD3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PLD3 Polyclonal antibody (17327-1-AP)

    WB analysis using 17327-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17327-1-AP (PLD3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PLD3 Polyclonal antibody (17327-1-AP)

    IHC staining of mouse brain using 17327-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17327-1-AP (PLD3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach tissue using PLD3 Polyclonal antibody (17327-1-AP)

    IHC staining of human stomach using 17327-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 17327-1-AP (PLD3 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach tissue using PLD3 Polyclonal antibody (17327-1-AP)

    IHC staining of human stomach using 17327-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 17327-1-AP (PLD3 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PLD3 Polyclonal antibody (17327-1-AP)

    IHC staining of mouse brain using 17327-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17327-1-AP (PLD3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using PLD3 Polyclonal antibody (17327-1-AP)

    IF Staining of mouse brain using 17327-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PLD3 antibody (17327-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using PLD3 Polyclonal antibody (17327-1-AP)

    IF Staining of mouse brain using 17327-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PLD3 antibody (17327-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using PLD3 Polyclonal antibody (17327-1-AP)

    IF Staining of A431 using 17327-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using PLD3 antibody (17327-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO Validated

PLD3 Polyclonal antibody

PLD3 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 17327-1-AP
Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Synonyms

5'-3' exonuclease PLD3, Choline phosphatase 3, EC:3.1.16.1, HindIII K4L homolog, HU K4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, SH-SY5Y cells, L02 cells, SK-N-SH cells
Positive IHC detected inhuman stomach tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17327-1-AP targets PLD3 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag11306

Product name: Recombinant human PLD3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 144-490 aa of BC036327

Sequence: GEEVLRQLQTLAPKGVNVRIAVSKPSGPQPQADLQALLQSGAQVRMVDMQKLTHGVLHTKFWVVDQTHFYLGSANMDWRSLTQVKELGVVMYNCSCLARDLTKIFEAYWFLGQAGSSIPSTWPRFYDTRYNQETPMEICLNGTPALAYLASAPPPLCPSGRTPDLKALLNVVDNARSFIYVAVMNYLPTLEFSHPHRFWPAIDDGLRRATYERGVKVRLLISCWGHSEPSMRAFLLSLAALRDNHTHSDIQVKLFVVPADEAQARIPYARVNHNKYMVTERATYIGTSNWSGNYFTETAGTSLLVTQNGRGGLRSQLEAIFLRDWDSPYSHDLDTSADSVGNACRLL

相同性解析による交差性が予測される生物種
Full Name phospholipase D family, member 3
Calculated molecular weight 490 aa, 55 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC036327
Gene Symbol PLD3
Gene ID (NCBI) 23646
RRIDAB_2878383
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IV08
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PLD3 antibody 17327-1-APDownload protocol
IHC protocol for PLD3 antibody 17327-1-APDownload protocol
IF protocol for PLD3 antibody 17327-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Hum Mol Genet

Pathological characterization of a novel mouse model expressing the PD-linked CHCHD2-T61I mutation.

Authors - Teresa R Kee
View Article
human

Cell Death Discov

A signature of enhanced lipid metabolism, lipid peroxidation and aldehyde stress in therapy-induced senescence.

Authors - Amy C Flor
View Article
mouseWB,IF

ACS Appl Mater Interfaces

FRET Nanosensor Based on DNA Tetrahedron for Visualizing PLD3 Fluctuation in Mouse Models of Alzheimer's Disease

Authors - Shuyan Wen
View Article