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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PLCZ1 Recombinant antibody

PLCZ1 Recombinant Antibody for FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

FC, ELISA

Conjugate

Unconjugated

CloneNo.

230187E2

Cat no : 83090-1-RR

Synonyms

NYD SP27, Phospholipase C zeta 1, phospholipase C, zeta 1, PLC zeta 1, PLCZ1, PLCzeta

Validation Data Gallery

Applications:
  • FC experiment of HEK-293 using 83090-1-RR

    FC experiment of HEK-293 using 83090-1-RR

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug Anti-Human PLCZ1 (83090-1-RR, Clone:230187E2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

Tested Applications

Positive FC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

83090-1-RR targets PLCZ1 in FC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PLCZ1 fusion protein Ag26899 相同性解析による交差性が予測される生物種
Full Name phospholipase C, zeta 1
Calculated molecular weight 608 aa, 70 kDa
GenBank accession numberBC125067
Gene symbol PLCZ1
Gene ID (NCBI) 89869
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Phospholipase C zeta1 (Plcz1) was known to be a physiological factor in sperm that activates oocytes to complete meiosis by triggering Ca2+ oscillations after fertilisation (PMID: 36613757). PLCZ1 is considered a major sperm-borne oocyte activation factor. After gamete fusion, PLCZ1 triggers calcium oscillations in the oocyte, resulting in oocyte activation (PMID: 38133249).