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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PLCL2 Monoclonal antibody

PLCL2 Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Mouse, Pig

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1C8A4

Cat no : 67471-1-Ig

Synonyms

FLJ13484, KIAA1092, Phospholipase C epsilon 2, Phospholipase C L2, phospholipase C like 2, PLC epsilon 2, PLC L(2), PLC L2, PLCE2, PLCL2

Validation Data Gallery

Applications:
  • WB analysis using 67471-1-Ig

    WB analysis using 67471-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67471-1-Ig (PLCL2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 67471-1-Ig

    IF Staining of HeLa using 67471-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 67471-1-Ig (PLCL2 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inpig brain tissue, mouse brain tissue, rat brain tissue
Positive IF detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

67471-1-Ig targets PLCL2 in WB, IF, ELISA applications and shows reactivity with Human, Rat, Mouse, Pig samples.

Tested Reactivity Human, Rat, Mouse, Pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PLCL2 fusion protein Ag11461 相同性解析による交差性が予測される生物種
Full Name phospholipase C-like 2
Calculated molecular weight 126 kDa
Observed molecular weight 126 kDa
GenBank accession numberBC036392
Gene symbol PLCL2
Gene ID (NCBI) 23228
RRIDAB_2882700
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PLCL2, a novel phospholipase C-like protein, is expressed in lymphocytes and platelets. The expression of PLCL2 is associated with the proliferation of mature B cells in the immune system. PLCL2 is a new susceptibility loci for myocardial infarction. It has been shown that PLCL2 plays a key role in the pathogenesis of atherosclerosis and systemic sclerosisit. There are three isoforms of PLCL2 protein and 67471-1-Ig antibody detects the 126 kDa band in SDS-PAGE. (PMID: 24916648, 25880423)

Protocols

Product Specific Protocols
WB protocol for PLCL2 antibody 67471-1-IgDownload protocol
IF protocol for PLCL2 antibody 67471-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols