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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse skeletal muscle tissue using PLCD4 Polyclonal antibody (10589-2-AP)

    WB analysis of mouse skeletal muscle using 10589-2-AP (same clone as 10589-2-PBS)

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10589-2-AP (PLCD4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10589-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PLCD4 Polyclonal antibody (10589-2-AP)

    WB analysis using 10589-2-AP (same clone as 10589-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10589-2-AP (PLCD4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10589-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using PLCD4 Polyclonal antibody (10589-2-AP)

    IHC staining of human pancreas cancer using 10589-2-AP (same clone as 10589-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10589-2-AP (PLCD4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10589-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using PLCD4 Polyclonal antibody (10589-2-AP)

    IHC staining of human pancreas cancer using 10589-2-AP (same clone as 10589-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10589-2-AP (PLCD4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10589-2-PBS in a different storage buffer formulation.

PLCD4 Polyclonal antibody, PBS Only

PLCD4 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 10589-2-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10589-2-PBS targets PLCD4 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag0937

Product name: Recombinant human PLCD4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 462-762 aa of BC006355

Sequence: ESQFETEPEPQEQNLQNKDKKKKSKPILCPALSSLVIYLKSVSFRSFTHSKEHYHFYEISSFSETKAKRLIKEAGNEFVQHNTWQLSRVYPSGLRTDSSNYNPQELWNAGCQMVAMNMQTAGLEMDICDGHFRQNGGCGYVLKPDFLRDIQSSFHPEKPISPFKAQTLLIQVISGQQLPKVDKTKEGSIVDPLVKVQIFGVRLDTARQETNYVENNGFNPYWGQTLCFRVLVPELAMLRFVVMDYDWKSRNDFIGQYTLPWTCMQQGYRHIHLLSKDGISLRPASIFVYICIQEGLEGDES

相同性解析による交差性が予測される生物種
Full Name phospholipase C, delta 4
Calculated molecular weight 85-90 kDa
Observed molecular weight100-110 kDa
GenBank accession numberBC006355
Gene Symbol PLCD4
Gene ID (NCBI) 84812
RRIDAB_2163398
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BRC7
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PLCD4 is a member of the delta class of phospholipase C enzymes. Phospholipase C enzymes play a critical role in many cellular processes by hydrolyzing phosphatidylinositol 4,5-bisphosphate into two intracellular second messengers, inositol 1,4,5-trisphosphate and diacylglycerol. Expression of this gene may be a marker for cancer.

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