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Validation Data Gallery

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  • Western Blot (WB) analysis of SH-SY5Y cells using PLCD3 Polyclonal antibody (16792-1-AP)

    WB analysis of SH-SY5Y using 16792-1-AP (same clone as 16792-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 16792-1-AP (PLCD3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16792-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U2OS cells using PLCD3 Polyclonal antibody (16792-1-AP)

    WB analysis of U2OS using 16792-1-AP (same clone as 16792-1-PBS)

    U2OS cells were subjected to SDS PAGE followed by western blot with 16792-1-AP (PLCD3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16792-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using PLCD3 Polyclonal antibody (16792-1-AP)

    WB analysis of A549 using 16792-1-AP (same clone as 16792-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 16792-1-AP (PLCD3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16792-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using PLCD3 Polyclonal antibody (16792-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 16792-1-AP (same clone as 16792-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 16792-1-AP (PLCD3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16792-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using PLCD3 Polyclonal antibody (16792-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 16792-1-AP (same clone as 16792-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 16792-1-AP (PLCD3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16792-1-PBS in a different storage buffer formulation.

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PLCD3 Polyclonal antibody, PBS Only

PLCD3 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 16792-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Phospholipase C-delta-3, phospholipase C, delta 3, Phospholipase C delta 3, Phosphoinositide phospholipase C-delta-3, EC:3.1.4.11

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16792-1-PBS targets PLCD3 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PLCD3 fusion protein Ag10249 相同性解析による交差性が予測される生物種
Full Name phospholipase C, delta 3
Calculated molecular weight 789 aa, 89 kDa
Observed molecular weight 85 kDa
GenBank accession numberBC072384
Gene Symbol PLCD3
Gene ID (NCBI) 113026
RRIDAB_2878316
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N3E9
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Phospholipase C Delta 3 (PLCD3) is a protein-coding gene located on chromosome 17 and a member of the phospholipase C family. PLCD3 is expressed broadly across different tissues, with high expression levels observed in the colon and urinary bladder, among others. The enzyme localizes to the plasma membrane and requires calcium for activation. Spermine, sphingosine, and several phospholipids can inhibit its activity. Recent studies have implicated PLCD3 in various types of cancer. In summary, PLCD3 is an important enzyme involved in cellular signaling and has been implicated in the development and progression of various cancers, making it a potential target for therapeutic interventions.