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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PLCB3 Recombinant antibody (85786-1-RR)

    WB analysis using 85786-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85786-1-RR (PLCB3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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PLCB3 Recombinant antibody

PLCB3 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 85786-1-RR
Clone No.243045B8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3, EC:3.1.4.11, Phosphoinositide phospholipase C-beta-3, Phospholipase C beta 3, Phospholipase C-beta-3

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, Jurkat cells, MCF-7 cells, U2OS cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85786-1-RR targets PLCB3 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name phospholipase C, beta 3 (phosphatidylinositol-specific)
Calculated molecular weight 1234 aa, 139 kDa
Observed molecular weight 139-150 kDa
GenBank accession numberBC142681
Gene Symbol PLCB3
Gene ID (NCBI) 5331
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ01970
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PLCB3 is a member of the phosphoinositide phospholipase C beta enzyme family that catalyze the production of the secondary messengers diacylglycerol and inositol 1,4,5-triphosphate from phosphatidylinositol in G-protein-linked receptor-mediated signal transduction. Six subfamilies of PLCs (B, G, D, E, Z and H) constitute part of ubiquitous signaling cascades that translate hormonal signals into intracellular events, leading to alternations in cell function. PLCB isoforms 1-4 are stimulated by G-protein activation (Gαq/11 and/or Gβγ). Independent of its enzymatic activity, PLCB3 inhibits the proliferation of hematopoietic stem cells (HSCs) and myeloid cells.

Protocols

Product Specific Protocols
WB protocol for PLCB3 antibody 85786-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols