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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using PLCB3 Polyclonal antibody (51139-1-AP)

    WB analysis of HeLa using 51139-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 51139-1-AP (PLCB3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293T cells using PLCB3 Polyclonal antibody (51139-1-AP)

    WB analysis of HEK-293T using 51139-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 51139-1-AP (PLCB3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using PLCB3 Polyclonal antibody (51139-1-AP)

    WB analysis of MCF-7 using 51139-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 51139-1-AP (PLCB3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of MDA-MB-231 cells using PLCB3 Polyclonal antibody (51139-1-AP)

    IP experiment of MDA-MB-231 using 51139-1-AP

    IP result of anti-PLCB3 (IP:51139-1-AP, 4ug; Detection:51139-1-AP 1:1000) with MDA-MB-231 cells lysate 1280 ug.

PLCB3 Polyclonal antibody

PLCB3 Polyclonal Antibody for WB, IP, ELISA
Cat No. 51139-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Synonyms

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3, EC:3.1.4.11, Phosphoinositide phospholipase C-beta-3, Phospholipase C beta 3, Phospholipase C-beta-3

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HEK-293T cells, MCF-7 cells
Positive IP detected inMDA-MB-231 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

51139-1-AP targets PLCB3 in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name phospholipase C, beta 3 (phosphatidylinositol-specific)
Calculated molecular weight 1234 aa, 139 kDa
Observed molecular weight 139 kDa
GenBank accession numberBC142681
Gene Symbol PLCB3
Gene ID (NCBI) 5331
RRIDAB_2881250
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ01970
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PLCB3 is a member of the phosphoinositide phospholipase C beta enzyme family that catalyze the production of the secondary messengers diacylglycerol and inositol 1,4,5-triphosphate from phosphatidylinositol in G-protein-linked receptor-mediated signal transduction. Six subfamilies of PLCs (B, G, D, E, Z and H) constitute part of ubiquitous signaling cascades that translate hormonal signals into intracellular events, leading to alternations in cell function. PLCB isoforms 1-4 are stimulated by G-protein activation (Gαq/11 and/or Gβγ). Independent of its enzymatic activity, PLCB3 inhibits the proliferation of hematopoietic stem cells (HSCs) and myeloid cells.

Protocols

Product Specific Protocols
IP protocol for PLCB3 antibody 51139-1-APDownload protocol
WB protocol for PLCB3 antibody 51139-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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