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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PLAP Recombinant monoclonal antibody (86752-3-RR)

    WB analysis using 86752-3-RR (same clone as 86752-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86752-3-RR (ALPP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86752-3-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PLAP Recombinant monoclonal antibody (86752-3-RR)

    WB analysis using 86752-3-RR (same clone as 86752-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86752-3-RR (ALPP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86752-3-PBS in a different storage buffer formulation.

  • Affinity of 86752-3-RR

    Affinity of 86752-3-RR

    Biolayer interferometry (BLl) kinetic assays of 86752-3-RR against Human PLAP were performed. The affinity constant is 2.06 nM.

PLAP Recombinant monoclonal antibody, PBS Only

PLAP Uni-rAb® Recombinant Antibody for WB, Indirect ELISA
Cat No. 86752-3-PBS
Clone No.251261A10

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, Indirect ELISA

Synonyms

ALPP, EC:3.1.3.1, PALP, PLAP-1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86752-3-PBS targets PLAP in WB, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg3065

Product name: Recombinant human ALPP protein (C-rFc)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 23-506 aa of NM_001632.4

Sequence: IIPVEEENPDFWNREAAEALGAAKKLQPAQTAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPEIPLAMDRFPYVALSKTYNVDKHVPDSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNRAKKAGKSVGVVTTTRVQHASPAGTYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVILGGGRKYMFRMGTPDPEYPDDYSQGGTRLDGKNLVQEWLAKRQGARYVWNRTELMQASLDPSVTHLMGLFEPGDMKYEIHRDSTLDPSLMEMTEAALRLLSRNPRGFFLFVEGGRIDHGHHESRAYRALTETIMFDDAIERAGQLTSEEDTLSLVTADHSHVFSFGGYPLRGSSIFGLAPGKARDRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEYRQQSAVPLDEETHAGEDVAVFARGPQAHLVHGVQEQTFIAHVMAFAACLEPYTACDLAPPAGTTD

相同性解析による交差性が予測される生物種
Full Name alkaline phosphatase, placental (Regan isozyme)
Calculated molecular weight58 kDa
Observed molecular weight 66 kDa
GenBank accession numberNM_001632.4
Gene Symbol PLAP
Gene ID (NCBI) 250
RRIDAB_3745102
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP05187
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Placental alkaline phosphatase (PLAP) is a glycosyl phosphatidylinositol (GPI)-anchored sialoglycoprotein , which shows greater resistance to heat inactivation than other alkaline phosphatase. Presented at high levels in placental trophoblasts, PLAP is recognized as an important marker of differentiation in human malignancies.

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