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Validation Data Gallery

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  • Western Blot (WB) analysis of human peripheral blood leukocyte using PLAC8 Polyclonal antibody (12284-1-AP)

    WB analysis of human peripheral blood leukocyte using 12284-1-AP (same clone as 12284-1-PBS)

    human peripheral blood leukocyte were subjected to SDS PAGE followed by western blot with 12284-1-AP (PLAC8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12284-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PLAC8 Polyclonal antibody (12284-1-AP)

    WB analysis using 12284-1-AP (same clone as 12284-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12284-1-AP (PLAC8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12284-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using PLAC8 Polyclonal antibody (12284-1-AP)

    IHC staining of human placenta using 12284-1-AP (same clone as 12284-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12284-1-AP (PLAC8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12284-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using PLAC8 Polyclonal antibody (12284-1-AP)

    IHC staining of human placenta using 12284-1-AP (same clone as 12284-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12284-1-AP (PLAC8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12284-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using PLAC8 Polyclonal antibody (12284-1-AP)

    IHC staining of human tonsillitis using 12284-1-AP (same clone as 12284-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12284-1-AP (PLAC8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12284-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using PLAC8 Polyclonal antibody (12284-1-AP)

    IHC staining of human tonsillitis using 12284-1-AP (same clone as 12284-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12284-1-AP (PLAC8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12284-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using PLAC8 Polyclonal antibody (12284-1-AP)

    IHC staining of human brain using 12284-1-AP (same clone as 12284-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 12284-1-AP (PLAC8 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12284-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using PLAC8 Polyclonal antibody (12284-1-AP)

    IHC staining of human brain using 12284-1-AP (same clone as 12284-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 12284-1-AP (PLAC8 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12284-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

PLAC8 Polyclonal antibody, PBS Only

PLAC8 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 12284-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

BM-004, C15, onzin, placenta 8, Placenta-specific gene 8 protein

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12284-1-PBS targets PLAC8 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2939

Product name: Recombinant human PLAC8 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-115 aa of BC012205

Sequence: MQAQAPVVVVTQPGVGPGPAPQNSNWQTGMCDCFSDCGVCLCGTFCFPCLGCQVAADMNECCLCGTSVAMRTLYRTRYGIPGSICDDYMATLCCPHCTLCQIKRDINRRRAMRTF

相同性解析による交差性が予測される生物種
Full Name placenta-specific 8
Calculated molecular weight 12 kDa
Observed molecular weight12 kDa
GenBank accession numberBC012205
Gene Symbol PLAC8
Gene ID (NCBI) 51316
RRIDAB_11182285
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NZF1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PLAC8, also named as Protein C15, is a placenta-specific protein. The expression was downregulated upon activation, particularly in dendritic cells generated in vitro from CD34 (142230) progenitor cells. The MW of PLAC8 is about 12-20kd.

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