PLA2G4A Monoclonal antibody
PLA2G4A Monoclonal Antibody for WB, IF, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
2D10H8
Cat no : 68133-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, HeLa cells, NIH/3T3 cells, A549 cells, K-562 cells |
Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
Sample-dependent, check data in validation data gallery |
Product Information
68133-1-Ig targets PLA2G4A in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse samples.
Tested Reactivity | Human, mouse |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | PLA2G4A fusion protein Ag32674 相同性解析による交差性が予測される生物種 |
Full Name | phospholipase A2, group IVA (cytosolic, calcium-dependent) |
Calculated molecular weight | 749 aa, 85 kDa |
Observed molecular weight | 85-100 kDa |
GenBank accession number | BC114340 |
Gene symbol | PLA2G4A |
Gene ID (NCBI) | 5321 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
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WB protocol for PLA2G4A antibody 68133-1-Ig | Download protocol |
IHC protocol for PLA2G4A antibody 68133-1-Ig | Download protocol |
IF protocol for PLA2G4A antibody 68133-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |