Validation Data Gallery
Tested Applications
| Positive WB detected in | human milk |
| Positive IP detected in | human milk |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17646-1-AP targets PLA2G3 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11845 Product name: Recombinant human PLA2G3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 160-509 aa of BC025316 Sequence: GVGDSAGNSSELGVFQGPDLCCREHDRCPQNISPLQYNYGIRNYRFHTISHCDCDTRFQQCLQNQHDSISDIVGVAFFNVLEIPCFVLEEQEACVAWYWWGGCRMYGTVPLARLQPRTFYNASWSSRATSPTPSSRSPAPPKPRQKQHLRKGPPHQKGSKRPSKANTTALQDPMVSPRLDVAPTGLQGPQGGLKPQGARWVCRSFRRHLDQCEHQIGPREIEFQLLNSAQEPLFHCNCTRRLARFLRLHSPPEVTNMLWELLGTTCFKLAPPLDCVEGKNCSRDPRAIRVSARHLRRLQQRRHQLQDKGTDERQPWPSEPLRGPMSFYNQCLQLTQAARRPDRQQKSWSQ 相同性解析による交差性が予測される生物種 |
| Full Name | phospholipase A2, group III |
| Calculated molecular weight | 509 aa, 57 kDa |
| Observed molecular weight | 57 kDa |
| GenBank accession number | BC025316 |
| Gene Symbol | PLA2G3 |
| Gene ID (NCBI) | 50487 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NZ20 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The PLA2G3 protein (full name: Phospholipase A2 Group III) is a member of the secreted phospholipase A2 (sPLA2) family. It is a calcium-dependent secretory protein whose primary biochemical function is to catalyze the hydrolysis of the sn-2 position of phospholipids, releasing arachidonic acid and lysophospholipids. It is an enzyme involved in the hydrolysis of membrane phospholipids and lipid metabolism, playing important roles in the development and progression of innate immunity, inflammatory diseases (such as asthma and psoriasis), atherosclerosis, and certain cancers (such as colorectal cancer and ovarian cancer).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for PLA2G3 antibody 17646-1-AP | Download protocol |
| IP protocol for PLA2G3 antibody 17646-1-AP | Download protocol |
| WB protocol for PLA2G3 antibody 17646-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |