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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse spleen tissue using LYPLA3 Polyclonal antibody (10863-2-AP)

    WB analysis of mouse spleen using 10863-2-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 10863-2-AP (LYPLA3 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using LYPLA3 Polyclonal antibody (10863-2-AP)

    WB analysis of mouse kidney using 10863-2-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10863-2-AP (LYPLA3 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using LYPLA3 Polyclonal antibody (10863-2-AP)

    IHC staining of human liver cancer using 10863-2-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10863-2-AP (LYPLA3 antibody) at dilution of 1:200 (under 40x lens)..

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using LYPLA3 Polyclonal antibody (10863-2-AP)

    IHC staining of human liver cancer using 10863-2-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10863-2-AP (LYPLA3 antibody) at dilution of 1:200 (under 10x lens)..

  • Immunohistochemistry (IHC) staining of human placenta tissue using LYPLA3 Polyclonal antibody (10863-2-AP)

    IHC staining of human placenta using 10863-2-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10863-2-AP (LYPLA3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using LYPLA3 Polyclonal antibody (10863-2-AP)

    IHC staining of mouse liver using 10863-2-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10863-2-AP (LYPLA3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

LYPLA3 Polyclonal antibody

LYPLA3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 10863-2-AP
Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Synonyms

PLA2G15, 1-O-acylceramide synthase, ACS, EC:2.3.1.-, EC:3.1.1.5

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse spleen tissue, mouse kidney tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10863-2-AP targets LYPLA3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1304

Product name: Recombinant human PLA2G15 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-277 aa of BC011640

Sequence: SYFHTMVESLVGWGYTRGEDVRGAPYDWRRAPNENGPYFLALREMIEEMYQLYGGPVVLVAHSMGNMYTLYFLQRQPQAWKDKYIRAFVSLGAPWGGVAKTLRVLASGDNNRIPVIGPLKIREQQRSAVSTSWLLPYNYTWSPEKVFVQTPTINYTLRDYRKFFQDIGFEDGWLMRQDTEGLVEATMPPGVQLHCLYGTGVPTPDSFYYESFPDRDPKICFGDGDGTVNLKSALQCQAWQSRQEHQVLLQELPGSEHIEMLANATTLAYLKRVLLGP

相同性解析による交差性が予測される生物種
Full Name phospholipase A2, group XV
Calculated molecular weight 47 kDa
Observed molecular weight 42-57 kDa
GenBank accession numberBC011640
Gene Symbol PLA2G15
Gene ID (NCBI) 23659
RRIDAB_2283832
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NCC3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PLA2G15 is also named as LYPLA3 and belongs to the AB hydrolase superfamily. It has transacylase and calcium-independent phospholipase A2 activity. PLA2G15 catalyzes the formation of 1-O-acyl-N-acetylsphingosine and the concomitant release of a lyso-phospholipid. It can be detected as 42-47 kDa and 57 kDa due to the N-glycosylated modification (PMID:11790796).

Protocols

Product Specific Protocols
IHC protocol for LYPLA3 antibody 10863-2-APDownload protocol
WB protocol for LYPLA3 antibody 10863-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

JOR Spine

Inhibition of PLA2G15 Alleviates Palmitic Acid-Induced Lysosomal Membrane Permeabilization in Human Nucleus Pulposus Cells During Intervertebral Disc Degeneration.

Authors - Liqun Duan
View Article
humanIF

bioRxiv

Loss of the lysosomal protein CLN3 modifies the lipid content of the nuclear envelope leading to DNA damage and activation of YAP1 pro-apoptotic signaling

Authors - Neuza Domingues
View Article

Cell Metab

Arachidonic acid triggers spermidine synthase secretion from primary tumor to induce skeletal muscle weakness upon irradiation

Authors - Xiaohui Zhang
View Article
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