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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PLA2G12B Polyclonal antibody (25799-1-AP)

    WB analysis using 25799-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25799-1-AP (PLA2G12B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using PLA2G12B Polyclonal antibody (25799-1-AP)

    WB analysis of HepG2 using 25799-1-AP

    WB result of PLA2G12B antibody (25799-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-PLA2G12B transfected HepG2 cells.

  • Western Blot (WB) analysis of HuH-7 cells using PLA2G12B Polyclonal antibody (25799-1-AP)

    WB analysis of HuH-7 using 25799-1-AP

    WB result of PLA2G12B antibody (25799-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-PLA2G12B transfected HuH-7 cells.

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  • Featured Product
  • KD/KO Validated

PLA2G12B Polyclonal antibody

PLA2G12B Polyclonal Antibody for WB, ELISA
Cat No. 25799-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

FKSG71, Group XIIB secretory phospholipase A2-like protein, Group XIII secretory phospholipase A2-like protein, GXIIB, GXIII sPLA2 like

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse liver tissue, HepG2 cells, HuH-7 cells, rat liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25799-1-AP targets PLA2G12B in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PLA2G12B fusion protein Ag22877 相同性解析による交差性が予測される生物種
Full Name phospholipase A2, group XIIB
Calculated molecular weight 195 aa, 22 kDa
Observed molecular weight21-22 kDa
GenBank accession numberBC111946
Gene Symbol PLA2G12B
Gene ID (NCBI) 84647
RRIDAB_3085823
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BX93
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Phospholipase A2 (PLA2) enzymes catalyze hydrolysis of glycolipids to release free fatty acids and lysophospholipids. PLA2G12B is a secreted group XIIB secretory phospholipase A2-like protein belonging to the PLA2 family, but it is catalytically inactive due to an amino acid change in its active site and has altered phospholipid-binding properties (PMID: 14516201). It is located outside the cell membranes and strongly expressed in liver, small intestine and kidney, and recently reports revealed that Pla2g12b gene may be implicated in HDL cholesterol levels and metabolism (PMID: 22912808). The calculated molecular weight of PLA2G12B is 21 kDa.

Protocols

Product Specific Protocols
WB protocol for PLA2G12B antibody 25799-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols