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Validation Data Gallery

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  • Western Blot (WB) analysis of RAW264.7 using PKNOX2 Polyclonal antibody (20352-1-AP)

    WB analysis of RAW264.7 using 20352-1-AP (same clone as 20352-1-PBS)

    RAW264.7 cells were subjected to SDS PAGE followed by western blot with 20352-1-AP (PKNOX2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20352-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using PKNOX2 Polyclonal antibody (20352-1-AP)

    WB analysis of mouse brain using 20352-1-AP (same clone as 20352-1-PBS)

    Mouse brain tissue were subjected to SDS PAGE followed by western blot with 20352-1-AP (PKNOX2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20352-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using PKNOX2 Polyclonal antibody (20352-1-AP)

    WB analysis of HeLa using 20352-1-AP (same clone as 20352-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 20352-1-AP (PKNOX2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20352-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse lung tissue using PKNOX2 Polyclonal antibody (20352-1-AP)

    WB analysis of mouse lung using 20352-1-AP (same clone as 20352-1-PBS)

    Mouse lung tissue were subjected to SDS PAGE followed by western blot with 20352-1-AP (PKNOX2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20352-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human skeletal muscle tissue using PKNOX2 Polyclonal antibody (20352-1-AP)

    WB analysis of human skeletal muscle using 20352-1-AP (same clone as 20352-1-PBS)

    Human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20352-1-AP (PKNOX2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20352-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using PKNOX2 Polyclonal antibody (20352-1-AP)

    IHC staining of human kidney using 20352-1-AP (same clone as 20352-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 20352-1-AP (PKNOX2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20352-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using PKNOX2 Polyclonal antibody (20352-1-AP)

    IHC staining of human kidney using 20352-1-AP (same clone as 20352-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 20352-1-AP (PKNOX2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20352-1-PBS in a different storage buffer formulation.

PKNOX2 Polyclonal antibody, PBS Only

PKNOX2 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 20352-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Homeobox protein PREP-2, PREP2

Formulation:  PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20352-1-PBS targets PKNOX2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag14195

Product name: Recombinant human PKNOX2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 350-471 aa of BC045626

Sequence: MLDASNPDPAPKAKKIKSQHRPTQRFWPNSIAAGVLQQQGGAPGTNPDGSINLDNLQSLSSDSATMAMQQAMMAAHDDSLDGTEEEDEDEMEEEEEELEEEVDELQTTNVSDLGLEHSDSLE

相同性解析による交差性が予測される生物種
Full Name PBX/knotted 1 homeobox 2
Calculated molecular weight 472 aa, 52 kDa
Observed molecular weight 70 kDa, 55 kDa
GenBank accession numberBC045626
Gene Symbol PKNOX2
Gene ID (NCBI) 63876
RRIDAB_10694277
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96KN3
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PKNOX2, PBX/knotted 1 homeobox 2, also named as PREP2, belongs to the three-amino acid loop extension (TALE) homeobox family. As it contains a DNA-binding motif, PKNOX2 may function as a nuclear transcription factor, experiments have reported the PKNOX2 protein locates to the nucleus. It was showed that highest expression of a 4-kb PREP2 (PKNOX2) transcript in heart, brain, skeletal muscle, and ovary(PMID:11972344). Several years later, PKNOX2 was identified as one of the cis-regulated genes for alcohol addiction in mice, however it has not been reported to be associated with any similar phenotype in humans to date (PMID:19721000). The molecular weight of the protein may differ from the theoretical value.

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