Validation Data Gallery

Filter: AllWBIHC

WB analysis of RAW264.7 using 20352-1-AP
RAW264.7 cells were subjected to SDS PAGE followed by western blot with 20352-1-AP (PKNOX2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse small intestine using 20352-1-AP
mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 20352-1-AP (PKNOX2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 20352-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 20352-1-AP (PKNOX2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 20352-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 20352-1-AP (PKNOX2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 20352-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 20352-1-AP (PKNOX2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of human skeletal muscle using 20352-1-AP
human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20352-1-AP (PKNOX2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of human kidney using 20352-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 20352-1-AP (PKNOX2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 20352-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 20352-1-AP (PKNOX2 antibody) at dilution of 1:50 (under 40x lens).

PKNOX2 Polyclonal antibody

PKNOX2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 20352-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF, RIP, IHC, ELISA

Synonyms

Homeobox protein PKNOX2, Homeobox protein PREP 2, PBX/knotted 1 homeobox 2, PBX/knotted homeobox 2, PKNOX2, PREP2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	RAW 264.7 cells, RAW264.7 cells
Positive IHC detected in	human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below
IF	See 1 publications below
RIP	See 1 publications below

Product Information

20352-1-AP targets PKNOX2 in WB, IF, RIP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	mouse, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag14195 Product name: Recombinant human PKNOX2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 350-471 aa of BC045626 Sequence: MLDASNPDPAPKAKKIKSQHRPTQRFWPNSIAAGVLQQQGGAPGTNPDGSINLDNLQSLSSDSATMAMQQAMMAAHDDSLDGTEEEDEDEMEEEEEELEEEVDELQTTNVSDLGLEHSDSLE 相同性解析による交差性が予測される生物種
Full Name	PBX/knotted 1 homeobox 2
Calculated molecular weight	472 aa, 52 kDa
Observed molecular weight	70 kDa, 55 kDa
GenBank accession number	BC045626
Gene Symbol	PKNOX2
Gene ID (NCBI)	63876
RRID	AB_10694277
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q96KN3
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PKNOX2, PBX/knotted 1 homeobox 2, also named as PREP2, belongs to the three-amino acid loop extension (TALE) homeobox family. As it contains a DNA-binding motif, PKNOX2 may function as a nuclear transcription factor, experiments have reported the PKNOX2 protein locates to the nucleus. It was showed that highest expression of a 4-kb PREP2 (PKNOX2) transcript in heart, brain, skeletal muscle, and ovary(PMID:11972344). Several years later, PKNOX2 was identified as one of the cis-regulated genes for alcohol addiction in mice, however it has not been reported to be associated with any similar phenotype in humans to date (PMID:19721000). The molecular weight of the protein may differ from the theoretical value.

Protocols

Product Specific Protocols
IHC protocol for PKNOX2 antibody 20352-1-AP	Download protocol
WB protocol for PKNOX2 antibody 20352-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	Biochem Biophys Res Commun PKNOX2 regulates myofibroblast functions and tubular cell survival during kidney fibrosis. Authors - Yoshiaki Miyake View Article
pig	WB,RIP	Int J Mol Sci LncRNA TCONS_00323213 Promotes Myogenic Differentiation by Interacting with PKNOX2 to Upregulate MyoG in Porcine Satellite Cells Authors - Mengxun Li View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of RAW264.7 using 20352-1-AP

WB analysis of mouse small intestine using 20352-1-AP

WB analysis of HeLa using 20352-1-AP

WB analysis of mouse brain using 20352-1-AP

WB analysis of mouse lung using 20352-1-AP

WB analysis of human skeletal muscle using 20352-1-AP

IHC staining of human kidney using 20352-1-AP