PKNOX1 Polyclonal antibody
PKNOX1 Polyclonal Antibody for WB, IF, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IP, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 10614-1-AP
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive WB detected in | SH-SY5Y cells, COLO 320 cells, HepG2 cells |
| Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 2 publications below |
| IHC | See 1 publications below |
| IP | See 1 publications below |
Product Information
10614-1-AP targets PKNOX1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PKNOX1 fusion protein Ag0939 相同性解析による交差性が予測される生物種 |
| Full Name | PBX/knotted 1 homeobox 1 |
| Calculated molecular weight | 48 kDa |
| Observed molecular weight | 50-55 kDa |
| GenBank accession number | BC007746 |
| Gene symbol | PKNOX1 |
| Gene ID (NCBI) | 5316 |
| RRID | AB_2164125 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PKNOX1 antibody 10614-1-AP | Download protocol |
| IHC protocol for PKNOX1 antibody 10614-1-AP | Download protocol |
| IF protocol for PKNOX1 antibody 10614-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Am J Hum Genet Gain-of-Function MN1 Truncation Variants Cause a Recognizable Syndrome with Craniofacial and Brain Abnormalities. | ||
