Validation Data Gallery
Tested Applications
| Positive WB detected in | MDA-MB-231 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16904-1-AP targets PKN3 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10518 Product name: Recombinant human PKN3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 559-889 aa of BC062558 Sequence: FRCLAVLGRGHFGKVLLVQFKGTGKYYAIKALKKQEVLSRDEIESLYCEKRILEAVGCTGHPFLLSLLACFQTSSHACFVTEFVPGGDLMMQIHEDVFPEPQARFYVACVVLGLQFLHEKKIIYRDLKLDNLLLDAQGFLKIADFGLCKEGIGFGDRTSTFCGTPEFLAPEVLTQEAYTRAVDWWGLGVLLYEMLVGECPFPGDTEEEVFDCIVNMDAPYPGFLSVQGLEFIQKLLQKCPEKRLGAGEQDAEEIKVQPFFRTTNWQALLARTIQPPFVPTLCGPADLRYFEGEFTGLPPALTPPAPHSLLTARQQAAFRDFDFVSERFLEP 相同性解析による交差性が予測される生物種 |
| Full Name | protein kinase N3 |
| Calculated molecular weight | 889 aa, 99 kDa |
| Observed molecular weight | 99 kDa |
| GenBank accession number | BC062558 |
| Gene Symbol | PKN3 |
| Gene ID (NCBI) | 29941 |
| RRID | AB_3741899 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6P5Z2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PKN3 encodes Protein Kinase N3, a serine/threonine protein kinase that acts in signal transduction and is involved in epithelial cell migration. It belongs to the AGC Ser/Thr protein kinase, PKC subfamily, and Protein kinase N family, placing it among enzymes that regulate phosphorylation-dependent signaling. Its predicted activity and pathway placement point to a role in intracellular signaling and protein phosphorylation.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PKN3 antibody 16904-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |