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Validation Data Gallery

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  • Western Blot (WB) analysis of KEK293 cells using PKM Polyclonal antibody (25659-1-AP)

    WB analysis of KEK293 cells using 25659-1-AP (same clone as 25659-1-PBS)

    WB result of PKM antibody (25659-1-AP, 1:3000) with si-control and si-PKM transfected HEK293 cell. This data was developed using the same antibody clone with 25659-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PKM Polyclonal antibody (25659-1-AP)

    WB analysis using 25659-1-AP (same clone as 25659-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25659-1-AP (PKM antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25659-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PKM Polyclonal antibody (25659-1-AP)

    WB analysis using 25659-1-AP (same clone as 25659-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25659-1-AP (PKM antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25659-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using PKM Polyclonal antibody (25659-1-AP)

    WB analysis of HEK-293 using 25659-1-AP (same clone as 25659-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25659-1-AP (PKM Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25659-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using PKM Polyclonal antibody (25659-1-AP)

    WB analysis of MCF-7 using 25659-1-AP (same clone as 25659-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 25659-1-AP (PKM Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25659-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using PKM Polyclonal antibody (25659-1-AP)

    WB analysis of COLO 320 using 25659-1-AP (same clone as 25659-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 25659-1-AP (PKM Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25659-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SMMC-7721 cells using PKM Polyclonal antibody (25659-1-AP)

    WB analysis of SMMC-7721 using 25659-1-AP (same clone as 25659-1-PBS)

    SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 25659-1-AP (PKM Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25659-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PKM Polyclonal antibody (25659-1-AP)

    IHC staining of human liver cancer using 25659-1-AP (same clone as 25659-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25659-1-AP (PKM Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 25659-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PKM Polyclonal antibody (25659-1-AP)

    IHC staining of human liver cancer using 25659-1-AP (same clone as 25659-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25659-1-AP (PKM Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 25659-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using PKM Polyclonal antibody (25659-1-AP)

    IHC staining of human colon using 25659-1-AP (same clone as 25659-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25659-1-AP (PKM antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25659-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PKM Polyclonal antibody (25659-1-AP)

    IF Staining of HeLa using 25659-1-AP (same clone as 25659-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PKM antibody (25659-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 25659-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PKM Polyclonal antibody (25659-1-AP)

    IF Staining of HeLa using 25659-1-AP (same clone as 25659-1-PBS)

    Immunofluorescent analysis of HeLa cells using 25659-1-AP (PKM antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25659-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

PKM Polyclonal antibody, PBS Only

PKM Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 25659-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25659-1-PBS targets PKM in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag20270

Product name: Recombinant human PKM protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-531 aa of BC000481

Sequence: MSKPHSEAGTAFIQTQQLHAAMADTFLEHMCRLDIDSPPITARNTGIICTIGPASRSVETLKEMIKSGMNVARLNFSHGTHEYHAETIKNVRTATESFASDPILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKQKGADFLVTEVENGGSLGSKKGVNLPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFASFIRKASDVHEVRKVLGEKGKNIKIISKIENHEGVRRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAIYHLQLFEELRRLAPITSDPTEATAVGAVEASFKCCSGAIIVLTKSGRSAHQVARYRPRAPIIAVTRNPQTARQAHLYRGIFPVLCKDPVQEAWAEDVDLRVNFAMNVGKARGFFKKGDVVIVLTGWRPGSGFTNTMRVVPVP

相同性解析による交差性が予測される生物種
Full Name PKM pyruvate kinase, muscle
Calculated molecular weight 58 kDa
Observed molecular weight 58 kDa
GenBank accession numberBC000481
Gene Symbol PKM
Gene ID (NCBI) 5315
RRIDAB_2880181
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP14618
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PKM, also named as OIP3, PK2, PK3, PK-M1/2, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM plays a general role in caspase independent cell death of tumor cells. PKM has 2 isoforms named as PKM1/M2. The activity of the M2 isoform (but not the M1 isoform) can be inhibited by tyrosine kinase signalling in tumourcells. The primary pyruvate kinase isoform before tumour development is PK-M1; however, the primary isoform from four independent tumours is PK-M2.(PMID:18337823). This antibody can recognize both PK-M1 and PK-M2.