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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using PKIB Polyclonal antibody (11942-1-AP)

    IHC staining of human stomach cancer using 11942-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11942-1-AP (PKIB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using PKIB Polyclonal antibody (11942-1-AP)

    IHC staining of human stomach cancer using 11942-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11942-1-AP (PKIB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PKIB Polyclonal antibody (11942-1-AP)

    IHC staining of mouse brain using 11942-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11942-1-AP (PKIB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PKIB Polyclonal antibody (11942-1-AP)

    IHC staining of mouse brain using 11942-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11942-1-AP (PKIB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using PKIB Polyclonal antibody (11942-1-AP)

    IHC staining of human gliomas using 11942-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas using 11942-1-AP (PKIB antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using PKIB Polyclonal antibody (11942-1-AP)

    IHC staining of mouse kidney using 11942-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11942-1-AP (PKIB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PKIB Polyclonal antibody

PKIB Polyclonal Antibody for IHC, ELISA
Cat No. 11942-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

PKI beta, PKIB, PRKACN2

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inhuman stomach cancer tissue, human gliomas tissue, mouse kidney tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

11942-1-AP targets PKIB in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2541

Product name: Recombinant human PKIB protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-78 aa of BC036011

Sequence: MRTDSSKMTDVESGVANFASSARAGRRNALPDIQSSAATDGTSDLPLKLEALSVKEDAKEKDEKTTQDQLEKPQNEEK

相同性解析による交差性が予測される生物種
Full Name protein kinase (cAMP-dependent, catalytic) inhibitor beta
Calculated molecular weight 78 aa, 9 kDa
GenBank accession numberBC036011
Gene Symbol PKIB
Gene ID (NCBI) 5570
RRIDAB_3085384
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9C010
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The cAMP-dependent protein kinase inhibitor-β (PKIB) is also named as PRKACN2. PKIB is presumed to be one of the regulatory factors controlling the cAMP-dependent protein kinase A signaling pathway (PMID: 23224602). PKIB plays an important role in regulating the proliferation, migration, and even metastasis of osteosarcoma (PMID: 17195088). The expression of PKIB in the cytoplasm of tumor is closely related to pAkt and the triple negative breast cancer (PMID: 28387904).

Protocols

Product Specific Protocols
IHC protocol for PKIB antibody 11942-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
MouseWB

Acta Pharmacol Sin

Asiatic acid alleviates ischemic myocardial injury in mice by modulating mitophagy- and glycophagy-based energy metabolism.

Authors - Fan Qiu
View Article
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