Validation Data Gallery
Tested Applications
| Positive WB detected in | LNCaP cells, HeLa cells, HEK-293 cells, Caki-1 cells |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
82524-2-RR targets PKD2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | polycystic kidney disease 2 (autosomal dominant) |
| Calculated molecular weight | 968 aa, 110 kDa |
| Observed molecular weight | 110 kDa |
| GenBank accession number | BC112263 |
| Gene Symbol | PKD2 |
| Gene ID (NCBI) | 5311 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q13563 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Polycystin 2 (PKD2), the product of the gene mutated in type 2 autosomal dominant polycystic kidney disease, belongs to the polycystin family. PKD2 is a ~110-kDa six-transmembrane channel protein with cytoplasmic N- and C-termini. This protein functions as a Ca2+-activated intracellular Ca2+ release channel in the endoplasmic reticulum. It is also present in the plasma membrane, where it functions as a nonselective cation channel. In addition, PKD2 expression has been documented in the primary cilium of kidney epithelial cells, where it is believed to have an essential role in mediating Ca2+ entry in response to flow rate changes, suggesting that it may be part of a mechanosensing machinery residing in the primary cilium. (PMID: 16135816; 10497221)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PKD2 antibody 82524-2-RR | Download protocol |
| WB protocol for PKD2 antibody 82524-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |