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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PKC Iota Monoclonal antibody (66493-1-Ig)

    WB analysis using 66493-1-Ig (same clone as 66493-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66493-1-Ig (PKC Iota antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66493-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using PKC Iota Monoclonal antibody (66493-1-Ig)

    WB analysis of HeLa using 66493-1-Ig (same clone as 66493-1-PBS)

    WB result of PKC Iota antibody (66493-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PKC Iota transfected HeLa cells. This data was developed using the same antibody clone with 66493-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PKC Iota Monoclonal antibody (66493-1-Ig)

    WB analysis using 66493-1-Ig (same clone as 66493-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66493-1-Ig (PKC iota antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66493-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using PKC Iota Monoclonal antibody (66493-1-Ig)

    WB analysis of MCF-7 using 66493-1-Ig (same clone as 66493-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66493-1-Ig (PKC iota antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66493-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using PKC Iota Monoclonal antibody (66493-1-Ig)

    WB analysis of HEK-293 using 66493-1-Ig (same clone as 66493-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66493-1-Ig (PKC iota antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66493-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of ROS1728 cells using PKC Iota Monoclonal antibody (66493-1-Ig)

    WB analysis of ROS1728 using 66493-1-Ig (same clone as 66493-1-PBS)

    ROS1728 cells were subjected to SDS PAGE followed by western blot with 66493-1-Ig (PKC iota antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66493-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of C6 cells using PKC Iota Monoclonal antibody (66493-1-Ig)

    WB analysis of C6 using 66493-1-Ig (same clone as 66493-1-PBS)

    C6 cells were subjected to SDS PAGE followed by western blot with 66493-1-Ig (PKC iota antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66493-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PKC Iota Monoclonal antibody (66493-1-Ig)

    IHC staining of human lung cancer using 66493-1-Ig (same clone as 66493-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66493-1-Ig (PKC iota antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66493-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PKC Iota Monoclonal antibody (66493-1-Ig)

    IHC staining of human lung cancer using 66493-1-Ig (same clone as 66493-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66493-1-Ig (PKC iota antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66493-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human lung cancer tissue using PKC Iota Monoclonal antibody (66493-1-Ig)

    IF Staining of human lung cancer using 66493-1-Ig (same clone as 66493-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using PKC Iota antibody (66493-1-Ig, Clone: 3A1B6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66493-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PKC Iota Monoclonal antibody (66493-1-Ig)

    IF Staining of HeLa using 66493-1-Ig (same clone as 66493-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PKC Iota antibody (66493-1-Ig, Clone: 3A1B6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66493-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

PKC Iota Monoclonal antibody, PBS Only

PKC Iota Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 66493-1-PBS
Clone No.3A1B6

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

PRKCI, 3A1B6, aPKC lambda/iota, aPKC-lambda/iota, Atypical protein kinase C-lambda/iota

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66493-1-PBS targets PKC Iota in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag4990

Product name: Recombinant human PRKCI protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 238-587 aa of BC022016

Sequence: SSLGLQDFDLLRVIGRGSYAKVLLVRLKKTDRIYAMKVVKKELVNDDEDIDWVQTEKHVFEQASNHPFLVGLHSCFQTESRLFFVIEYVNGGDLMFHMQRQRKLPEEHARFYSAEISLALNYLHERGIIYRDLKLDNVLLDSEGHIKLTDYGMCKEGLRPGDTTSTFCGTPNYIAPEILRGEDYGFSVDWWALGVLMFEMMAGRSPFDIVGSSDNPDQNTEDYLFQVILEKQIRIPRSMSVKAASVLKSFLNKDPKERLGCLPQTGFADIQGHPFFRNVDWDMMEQKQVVPPFKPNISGEFGLDNFDSQFTNERVQLTPDDDDIVRKIDQSEFEGFEYINPLLMSAEECV

相同性解析による交差性が予測される生物種
Full Name protein kinase C, iota
Calculated molecular weight 68 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC022016
Gene Symbol PKC Iota
Gene ID (NCBI) 5584
RRIDAB_2881858
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP41743
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The atypical protein kinase C isoform PRKC iota (PRKCI) is a member of the protein kinase C (PKC) family of serine/threonine protein kinases. PKC family comprises at least eight members, which are differentially expressed and are involved in a wide variety of cellular processes. PRKC iota is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. PRKC iota plays a key role in cell proliferation, differentiation, and carcinogenesis, and it has been shown to be a human oncogene.