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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PKC Gamma Monoclonal antibody (66429-1-Ig)

    WB analysis using 66429-1-Ig (same clone as 66429-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66429-1-Ig (PKC gamma antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66429-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using PKC Gamma Monoclonal antibody (66429-1-Ig)

    IHC staining of human brain using 66429-1-Ig (same clone as 66429-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66429-1-Ig (PKC gamma antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66429-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using PKC Gamma Monoclonal antibody (66429-1-Ig)

    IHC staining of human brain using 66429-1-Ig (same clone as 66429-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66429-1-Ig (PKC gamma antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66429-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using PKC Gamma Monoclonal antibody (66429-1-Ig)

    IF Staining of SH-SY5Y using 66429-1-Ig (same clone as 66429-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed SH-SY5Y cells using 66429-1-Ig(PKC gamma antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66429-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of SH-SY5Y cells using PKC Gamma Monoclonal antibody (66429-1-Ig)

    FC experiment of SH-SY5Y using 66429-1-Ig (same clone as 66429-1-PBS)

    1X10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug Anti-Human PKC Gamma (66429-1-Ig, Clone:2F4B9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66429-1-PBS in a different storage buffer formulation.

PKC Gamma Monoclonal antibody, PBS Only

PKC Gamma Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 66429-1-PBS
Clone No.2F4B9

Host / Isotype

Mouse / IgG2a

Reactivity

pig, rat, mouse, human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

PRKCG, PKC-gamma, PKCG, PKCC, EC:2.7.11.13

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66429-1-PBS targets PKC Gamma in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with pig, rat, mouse, human samples.

Tested Reactivity pig, rat, mouse, human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag5910

Product name: Recombinant human PRKCG protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 351-697 aa of BC047876

Sequence: FSFLMVLGKGSFGKVMLAERRGSDELYAIKILKKDVIVQDDDVDCTLVEKRVLALGGRGPGGRPHFLTQLHSTFQTPDRLYFVMEYVTGGDLMYHIQQLGKFKEPHAAFYAAEIAIGLFFLHNQGIIYRDLKLDNVMLDAEGHIKITDFGMCKENVFPGTTTRTFCGTPDYIAPEIIAYQPYGKSVDWWSFGVLLYEMLAGQPPFDGEDEEELFQAIMEQTVTYPKSLSREAVAICKGFLTKHPGKRLGSGPDGEPTIRAHGFFRWIDWERLERLEIPPPFRPRPCGRSGENFDKFFTRAAPALTPPDRLVLASIDQADFQGFTYVNPDFVHPDARSPTSPVPVPVM

相同性解析による交差性が予測される生物種
Full Name protein kinase C, gamma
Calculated molecular weight 78 kDa
Observed molecular weight 78 kDa
GenBank accession numberBC047876
Gene Symbol PKC Gamma
Gene ID (NCBI) 5582
RRIDAB_2881800
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP05129
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC gamma is a neuron-specific member of the classical PKCs and is activated and translocated to subcellular regions as a result of various stimuli, including diacylglycerol synthesis, increased intracellular Ca(2+) and phorbol esters. Defects in this protein have been associated with spinocerebellar ataxia type 14 (SCA14), an autosomal dominant neurodegenerative disease.