Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IHC detected in | mouse cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IF | See 1 publications below |
Product Information
29208-1-AP targets PKC Gamma in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.
| Tested Reactivity | Human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag25085 Product name: Recombinant human PRKCG protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 600-641 aa of BC047876 Sequence: SGPDGEPTIRAHGFFRWIDWERLERLEIPPPFRPRPCGRSGE 相同性解析による交差性が予測される生物種 |
| Full Name | protein kinase C, gamma |
| Calculated molecular weight | 78 kDa |
| Observed molecular weight | 78 kDa |
| GenBank accession number | BC047876 |
| Gene Symbol | PKC Gamma |
| Gene ID (NCBI) | 5582 |
| RRID | AB_2918249 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P05129 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Background Information
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC gamma is a neuron-specific member of the classical PKCs and is activated and translocated to subcellular regions as a result of various stimuli, including diacylglycerol synthesis, increased intracellular Ca(2+) and phorbol esters. Defects in this protein have been associated with spinocerebellar ataxia type 14 (SCA14), an autosomal dominant neurodegenerative disease.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for PKC Gamma antibody 29208-1-AP | Download protocol |
| WB protocol for PKC Gamma antibody 29208-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cereb Cortex Protein phosphatase 2A deficiency in hippocampal CA1 inhibits priming effect of morphine on conditioned place preference in mice | ||
Gene Pathogenicity analysis of three SCA14-associated missense mutations in PRKCG gene of Chinese patients with ataxia |
