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Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using PKC Delta Recombinant antibody (84261-5-RR)

    WB analysis of NIH/3T3 using 84261-5-RR

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 84261-5-RR (PKC Delta antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PKC Delta Recombinant antibody (84261-5-RR)

    WB analysis using 84261-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84261-5-RR (PKC Delta antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PKC Delta Recombinant antibody (84261-5-RR)

    IF Staining of HeLa using 84261-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PRKCD antibody (84261-5-RR, Clone: 241608A2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HT-1080 cells using PKC Delta Recombinant antibody (84261-5-RR)

    FC experiment of HT-1080 using 84261-5-RR

    1x10^6 HT-1080 cells were intracellularly stained with 0.25 ug PRKCD Recombinant Antibody (84261-5-RR, Clone:241608A2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Flow cytometry (FC) experiment of Hela cells using PKC Delta Recombinant antibody (84261-5-RR)

    FC experiment of Hela using 84261-5-RR

    1x10^6 Hela cells were intracellularly stained with 0.25 ug PRKCD Recombinant Antibody (84261-5-RR, Clone:241608A2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Affinity and Kinetic Characterization of 84261-5-RR

    Affinity and Kinetic Characterization of 84261-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84261-5-RR against Human PKC Delta were performed. The affinity constant is 0.437 nM.

PKC Delta Recombinant monoclonal antibody

PKC Delta Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 84261-5-RR
Clone No.241608A2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

PRKCD, 241608A2, EC:2.7.10.2, EC:2.7.11.13, MAY1

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inNIH/3T3 cells, mouse brain tissue, rat brain tissue
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHT-1080 cells, Hela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84261-5-RR targets PKC Delta in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag5473

Product name: Recombinant human PRKCD protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 326-676 aa of BC043350

Sequence: DMQDNSGTYGKIWEGSSKCNINNFIFHKVLGKGSFGKVLLGELKGRGEYFAIKALKKDVVLIDDDVECTMVEKRVLTLAAENPFLTHLICTFQTKDHLFFVMEFLNGGDLMYHIQDKGRFELYRATFYAAEIMCGLQFLHSKGIIYRDLKLDNVLLDRDGHIKIADFGMCKENIFGESRASTFCGTPDYIAPEILQGLKYTFSVDWWSFGVLLYEMLIGQSPFHGDDEDELFESIRVDTPHYPRWITKESKDILEKLFEREPTKRLGVTGNIKIHPFFKTINWTLLEKRRLEPPFRPKVKSPRDYSNFDQEFLNEKARLSYSDKNLIDSMDQSAFAGFSFVNPKFEHLLED

相同性解析による交差性が予測される生物種
Full Name protein kinase C, delta
Calculated molecular weight 78 kDa
Observed molecular weight 70-80 kDa
GenBank accession numberBC043350
Gene Symbol PKC Delta
Gene ID (NCBI) 5580
RRIDAB_3671808
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ05655
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PKC Delta is a member of the protein kinase C family of serine- and threonine-specific protein kinases. The encoded protein is activated by diacylglycerol and is both a tumor suppressor and a positive regulator of cell cycle progression. Also, this protein can positively or negatively regulate apoptosis. Defects in this gene are a cause of autoimmune lymphoproliferative syndrom.

Protocols

Product Specific Protocols
IF protocol for PKC Delta antibody 84261-5-RRDownload protocol
WB protocol for PKC Delta antibody 84261-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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