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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PJA1 Polyclonal antibody (17687-1-AP)

    WB analysis using 17687-1-AP (same clone as 17687-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17687-1-AP (PJA1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17687-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using PJA1 Polyclonal antibody (17687-1-AP)

    WB analysis of mouse brain using 17687-1-AP (same clone as 17687-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 17687-1-AP (PJA1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17687-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using PJA1 Polyclonal antibody (17687-1-AP)

    WB analysis of rat brain using 17687-1-AP (same clone as 17687-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 17687-1-AP (PJA1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17687-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using PJA1 Polyclonal antibody (17687-1-AP)

    WB analysis of mouse brain using 17687-1-AP (same clone as 17687-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 17687-1-AP (PJA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17687-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using PJA1 Polyclonal antibody (17687-1-AP)

    IP experiment of mouse brain using 17687-1-AP (same clone as 17687-1-PBS)

    IP result of anti-PJA1 (IP:17687-1-AP, 3ug; Detection:17687-1-AP 1:300) with mouse brain tissue lysate 3600ug. This data was developed using the same antibody clone with 17687-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

PJA1 Polyclonal antibody, PBS Only

PJA1 Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 17687-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, Indirect ELISA

Synonyms

E3 ubiquitin-protein ligase Praja-1, EC:2.3.2.27, praja ring finger 1, Praja1, RING finger protein 70

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17687-1-PBS targets PJA1 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag11820

Product name: Recombinant human PJA1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 294-643 aa of BC075803

Sequence: ANDNEGHSDGLARRGRGESSSGYPEPKYPEDKREARSDQVKPEKVPRRRRTMADPDFWTHSDDYYKYCDEDSDSDKEWIAALRRKYRSREQTLSSSGESWETLPGKEEREPPQAKVSASTGTSPGPGASASAGAGAGASAGSNGSNYLEEVREPSLQEEQASLEEGEIPWLQYHENDSSSEGDNDSGHELMQPGVFMLDGNNNLEDDSSVSEDLEVDWSLFDGFADGLGVAEAISYVDPQFLTYMALEERLAQAMETALAHLESLAVDVEVANPPASKESIDALPEILVTEDHGAVGQEMCCPICCSEYVKGEVATELPCHHYFHKPCVSIWLQKSGTCPVCRCMFPPPL

相同性解析による交差性が予測される生物種
Full Name praja ring finger 1
Calculated molecular weight 643 aa, 71 kDa
Observed molecular weight 71-80 kDa
GenBank accession numberBC075803
Gene Symbol PJA1
Gene ID (NCBI) 64219
RRIDAB_2252483
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NG27
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PJA1(E3 ubiquitin-protein ligase Praja-1), also named as RNF70, regulates the level of PRC2 (polycomb repressive complex 2) by targeting its free subunits for Ub-mediated proteasomal degradation and it can be involved in protein ubiquitination in the brain and is a suitable candidate gene for MRX(PMID:12036302). The Praja1 protein is unlikely to be a membrane receptor, since it lacks a hydrophobic transmembrane domain(PMID:9393880). It has 3 isoforms produced by alternative splicing with the molecular weight of 71 kDa, 50 kDa and 65 kDa.

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