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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PIWIL4 Polyclonal antibody (12966-1-AP)

    WB analysis using 12966-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12966-1-AP (PIWIL4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

PIWIL4 Polyclonal antibody

PIWIL4 Polyclonal Antibody for WB, ELISA
Cat No. 12966-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

HIWI2, MIWI2, PIWI, piwi like 4 (Drosophila), Piwi like protein 4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman testis tissue, mouse testis tissue, rat testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12966-1-AP targets PIWIL4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PIWIL4 fusion protein Ag3613 相同性解析による交差性が予測される生物種
Full Name piwi-like 4 (Drosophila)
Calculated molecular weight 852 aa, 97 kDa
Observed molecular weight 97 kDa
GenBank accession numberBC031060
Gene Symbol PIWIL4
Gene ID (NCBI) 143689
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z3Z4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PIWIL4 (PiWI-like RNA-mediated gene silencing 4) is a member of the PIWI protein family and is mainly involved in the development and maintenance of reproductive stem cells. PIWIL4 regulates the biosynthesis and function of piRNA in germ cells by binding to PIWI-interacting RNA (piRNA). Furthermore, PIWIL4 is abnormally expressed in a variety of cancers and plays a promoting role in cancer.

Protocols

Product Specific Protocols
WB protocol for PIWIL4 antibody 12966-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols