Validation Data Gallery
Tested Applications
| Positive WB detected in | A431 cells, MCF-7 cells, HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human tonsillitis tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 9 publications below |
| IHC | See 3 publications below |
| IF | See 2 publications below |
Product Information
10873-1-AP targets PITX1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1303 Product name: Recombinant human PITX1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-314 aa of BC009412 Sequence: MDAFKGGMSLERLPEGLRPPPPPPHDMGPAFHLARPADPREPLENSASESSDTELPEKERGGEPKGPEDSGAGGTGCGGADDPAKKKKQRRQRTHFTSQQLQELEATFQRNRYPDMSMREEIAVWTNLTEPRVRVWFKNRRAKWRKRERNQQLDLCKGGYVPQFSGLVQPYEDVYAAGYSYNNWAAKSLAPAPLSTKSFTFFNSMSPLSSQSMFSAPSSISSMTMPSSMGPGAVPGMPNSGLNNINNLTGSSLNSAMSPGACPYGTPASPYSVYRDTCNSSLASLRLKSKQHSSFGYGGLQGPASGLNACQYNS 相同性解析による交差性が予測される生物種 |
| Full Name | paired-like homeodomain 1 |
| Calculated molecular weight | 34 kDa |
| Observed molecular weight | 38-40 kDa |
| GenBank accession number | BC009412 |
| Gene Symbol | PITX1 |
| Gene ID (NCBI) | 5307 |
| RRID | AB_10859664 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P78337 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PITX1 (Paired-like homeodomain 1) is a bicoid-related homeobox transcription factor that plays critical roles in embryonic development, particularly in patterning the hindlimb and regulating pituitary organogenesis. It is also known as "Backfoot" due to its involvement in establishing left-right asymmetry during limb formation. Beyond development, PITX1 has been implicated in various cancers, where it can function as either a tumor suppressor or an oncogene depending on the cellular context, and its dysregulation is associated with tumor progression and metastasis.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for PITX1 antibody 10873-1-AP | Download protocol |
| IP protocol for PITX1 antibody 10873-1-AP | Download protocol |
| WB protocol for PITX1 antibody 10873-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res G3BP1 binds to guanine quadruplexes in mRNAs to modulate their stabilities. | ||
Hum Mutat Mandibular-pelvic-patellar syndrome is a novel PITX1-related disorder due to alteration of PITX1 transactivation ability. | ||
Endocrinology Proton Sensitivity of Corticotropin-Releasing Hormone Receptor 1 Signaling to Proopiomelanocortin in Male Mice. | ||
Int J Biochem Cell Biol Paired like homeodomain 1 and SAM and SH3 domain-containing 1 in the progression and prognosis of head and neck squamous cell carcinoma.
| ||
Toxicol Lett All-trans-retinoid acid (ATRA) may have inhibited chondrogenesis of primary hind limb bud mesenchymal cells by downregulating Pitx1 expression. |