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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse lung tissue using PITPNB Polyclonal antibody (13110-1-AP)

    WB analysis of mouse lung using 13110-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 13110-1-AP (PITPNB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PITPNB Polyclonal antibody (13110-1-AP)

    WB analysis using 13110-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13110-1-AP (PITPNB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using PITPNB Polyclonal antibody (13110-1-AP)

    WB analysis of K-562 using 13110-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 13110-1-AP (PITPNB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PITPNB Polyclonal antibody (13110-1-AP)

    WB analysis of HeLa using 13110-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 13110-1-AP (PITPNB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using PITPNB Polyclonal antibody (13110-1-AP)

    WB analysis of Raji using 13110-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 13110-1-AP (PITPNB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using PITPNB Polyclonal antibody (13110-1-AP)

    IHC staining of human prostate cancer using 13110-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13110-1-AP (PITPNB antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using PITPNB Polyclonal antibody (13110-1-AP)

    IHC staining of human prostate cancer using 13110-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13110-1-AP (PITPNB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using PITPNB Polyclonal antibody (13110-1-AP)

    IHC staining of human prostate cancer using 13110-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13110-1-AP (PITPNB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO Validated

PITPNB Polyclonal antibody

PITPNB Polyclonal Antibody for WB, IHC, ELISA
Cat No. 13110-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

PI TP beta, PITPNB, PtdIns transfer protein beta, PtdInsTP, PtdInsTP beta, VIB1B

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inK-562 cells, mouse lung tissue, HeLa cells, Raji cells, rat lung tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13110-1-AP targets PITPNB in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3774

Product name: Recombinant human PITPNB protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-272 aa of BC031427

Sequence: MVLIKEFRVVLPCSVQEYQVGQLYSVAEASKNETGGGEGIEVLKNEPYEKDGEKGQYTHKIYHLKSKVPAFVRMIAPEGSLVFHEKAWNAYPYCRTIVTNEYMKDDFFIKIETWHKPDLGTLENVHGLDPNTWKTVEIVHIDIADRSQVEPADYKADEDPALFQSVKTKRGPLGPNWKKELANSPDCPQMCAYKLVTIKFKWWGLQSKVENFIQKQEKRIFTNFHRQLFCWIDKWIDLTMEDIRRMEDETQKELETLRNQGQVRGTSAASDE

相同性解析による交差性が予測される生物種
Full Name phosphatidylinositol transfer protein, beta
Calculated molecular weight 272 aa, 32 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC031427
Gene Symbol PITPNB
Gene ID (NCBI) 23760
RRIDAB_2164999
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP48739
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for PITPNB antibody 13110-1-APDownload protocol
WB protocol for PITPNB antibody 13110-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Virol

Nir2 Is an Effector of VAPs Necessary for Efficient Hepatitis C Virus Replication and Phosphatidylinositol 4-Phosphate Enrichment at the Viral Replication Organelle.

Authors - Hongliang Wang
  • KO Validated
View Article
humanWB

Elife

The ORP9-ORP11 dimer promotes sphingomyelin synthesis

Authors - Birol Cabukusta
  • KO Validated
View Article
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