Validation Data Gallery

Filter: AllWBIPIHC

WB analysis of K-562 using 16401-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 16401-1-AP (PISD antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis using 16401-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16401-1-AP (PISD antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 16401-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16401-1-AP (PISD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of L02 using 16401-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 16401-1-AP (PISD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of L02 using 16401-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 16401-1-AP (PISD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of L02 using 16401-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 16401-1-AP (PISD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human spleen using 16401-1-AP
human spleen tissue were subjected to SDS PAGE followed by western blot with 16401-1-AP (PISD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human kidney using 16401-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 16401-1-AP (PISD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 16401-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 16401-1-AP (PISD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 16401-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 16401-1-AP (PISD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 16401-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 16401-1-AP (PISD antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of L02 using 16401-1-AP
IP result of anti-PISD (IP:16401-1-AP, 3ug; Detection:16401-1-AP 1:500) with L02 cells lysate 1400ug.
IHC staining of human kidney using 16401-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 16401-1-AP (PISD antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human kidney using 16401-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 16401-1-AP (PISD antibody) at dilution of 1:100 (under 40x lens).

Featured Product
KD/KO Validated

PISD Polyclonal antibody

PISD Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 16401-1-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

PSSC, PSDC, Phosphatidylserine decarboxylase proenzyme, mitochondrial, Phosphatidylserine decarboxylase beta chain, Phosphatidylserine decarboxylase alpha chain

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (6)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, COLO 320 cells, HepG2 cells, human kidney tissue, human spleen tissue, K-562 cells, L02 cells, NIH/3T3 cells
Positive IP detected in	L02 cells
Positive IHC detected in	human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 5 publications below

Product Information

16401-1-AP targets PISD in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag9499 Product name: Recombinant human PISD protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-375 aa of BC001482 Sequence: MMCQSEARQGPELRAAKWLHFPQLALRRRLGQLSCMSRPALKLRSWPLTVLYYLLPFGALRPLSRVGWRPVSRVALYKSVPTRLLSRAWGRLNQVELPHWLRRPVYSLYIWTFGVNMKEAAVEDLHHYRNLSEFFRRKLKPQARPVCGLHSVISPSDGRILNFGQVKNCEVEQVKGVTYSLESFLGPRMCTEDLPFPPAASCDSFKNQLVTREGNELYHCVIYLAPGDYHCFHSPTDWTVSHRRHFPGSLMSVNPGMARWIKELFCHNERVVLTGDWKHGFFSLTAVGATNVGSIRIYFDRDLHTNSPRHSKGSYNDFSFVTHTNREGVPMRKGEHLGEFNLGSTIVLIFEAPKDFNFQLKTGQKIRFGEALGSL 相同性解析による交差性が予測される生物種
Full Name	phosphatidylserine decarboxylase
Calculated molecular weight	375aa,43 kDa; 408aa,46 kDa
Observed molecular weight	45~55 kDa
GenBank accession number	BC001482
Gene Symbol	PISD
Gene ID (NCBI)	23761
RRID	AB_2186673
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9UG56
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PISD(Phosphatidylserine decarboxylase proenzyme) belongs to the phosphatidylserine decarboxylase family.It contains a conserved LGST motif that functions as an autocatalytic cleavage site where the proenzyme is split into mature alpha and beta subunits.It has 2 isoforms produced by alternative splicing.This antibody is specific to PISD. The sequence correspond to the beta isoform.

Protocols

Product Specific Protocols
IHC protocol for PISD antibody 16401-1-AP	Download protocol
IP protocol for PISD antibody 16401-1-AP	Download protocol
WB protocol for PISD antibody 16401-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell Death Dis Aberrant ER-mitochondria communication is a common pathomechanism in mitochondrial disease Authors - Patricia Morcillo View Article
human	WB	Cancer Lett LACTB suppresses melanoma progression by attenuating PP1A and YAP interaction. Authors - Yawen Ma View Article
human		Front Cell Dev Biol Screening and diagnosis of triple negative breast cancer based on rapid metabolic fingerprinting by conductive polymer spray ionization mass spectrometry and machine learning Authors - Yaoyao Song View Article
human	WB	J Clin Biochem Nutr Metabolic changes induced by TGF-β1 via reduced expression of phosphatidylserine decarboxylase during myofibroblast transition Authors - Nobuhiko Uchida View Article
mouse	WB	bioRxiv Distinct yet Overlapping Functions of VMP1 and TMEM41B in Modulating Hepatic Lipoprotein Secretion and Autophagy in MASH Authors - Allen Chen View Article
human	WB	Cell Stem Cell The Mitochondrial Transacylase, Tafazzin, Regulates for AML Stemness by Modulating Intracellular Levels of Phospholipids. Authors - Ayesh K Seneviratne KO Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of K-562 using 16401-1-AP

WB analysis using 16401-1-AP

WB analysis of NIH/3T3 using 16401-1-AP

WB analysis of L02 using 16401-1-AP

WB analysis of L02 using 16401-1-AP

WB analysis of L02 using 16401-1-AP

WB analysis of human spleen using 16401-1-AP

WB analysis of human kidney using 16401-1-AP

WB analysis of COLO 320 using 16401-1-AP

WB analysis of HeLa using 16401-1-AP

WB analysis of HepG2 using 16401-1-AP

IP experiment of L02 using 16401-1-AP

IHC staining of human kidney using 16401-1-AP

IHC staining of human kidney using 16401-1-AP

PISD Polyclonal antibody

PISD Polyclonal Antibody for WB, IHC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Aberrant ER-mitochondria communication is a common pathomechanism in mitochondrial disease

LACTB suppresses melanoma progression by attenuating PP1A and YAP interaction.

Screening and diagnosis of triple negative breast cancer based on rapid metabolic fingerprinting by conductive polymer spray ionization mass spectrometry and machine learning

Metabolic changes induced by TGF-β1 via reduced expression of phosphatidylserine decarboxylase during myofibroblast transition

Distinct yet Overlapping Functions of VMP1 and TMEM41B in Modulating Hepatic Lipoprotein Secretion and Autophagy in MASH

The Mitochondrial Transacylase, Tafazzin, Regulates for AML Stemness by Modulating Intracellular Levels of Phospholipids.