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Validation Data Gallery

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  • Western Blot (WB) analysis of HSC-T6 cells using PIR Monoclonal antibody (67516-1-Ig)

    WB analysis of HSC-T6 using 67516-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67516-1-Ig (PIR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using PIR Monoclonal antibody (67516-1-Ig)

    WB analysis of NIH/3T3 using 67516-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67516-1-Ig (PIR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PIR Monoclonal antibody (67516-1-Ig)

    WB analysis of HeLa using 67516-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 67516-1-Ig (PIR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using PIR Monoclonal antibody (67516-1-Ig)

    WB analysis of K-562 using 67516-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 67516-1-Ig (PIR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

PIR Monoclonal antibody

PIR Monoclonal Antibody for WB, ELISA
Cat No. 67516-1-Ig
Clone No.2A10E4

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Synonyms

PIR, Pirin

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHSC-T6 cells, HeLa cells, K-562 cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67516-1-Ig targets PIR in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag29969

Product name: Recombinant human PIR protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 46-230 aa of BC002517

Sequence: KGGRPGGFPDHPHRGFETVSYLLEGGSMAHEDFCGHTGKMNPGDLQWMTAGRGILHAEMPCSEEPAHGLQLWVNLRSSEKMVEPQYQELKSEEIPKPSKDGVTVAVISGEALGIKSKVYTRTPTLYLDFKLDPGAKHSQPIPKGWTSFIYTISGDVYIGPDDAQQKIEPHHTAVLGEGDSVQVEN

相同性解析による交差性が予測される生物種
Full Name pirin (iron-binding nuclear protein)
Calculated molecular weight 32 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC002517
Gene Symbol PIR
Gene ID (NCBI) 8544
RRIDAB_2882737
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDO00625
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Pirin (iron-binding nuclear protein, PIR) is a highly conserved 32-kDa protein consisting of 290 amino acids. Pirin mRNA is expressed weakly in all human tissues. Confocal immunofluorescence experiments demonstrate a predominant localization of Pirin within dot-like subnuclear structures. Pirin interacts with the oncoprotein B-cell lymphoma 3-encoded (Bcl-3) and nuclear factor I (NFI).

Protocols

Product Specific Protocols
WB protocol for PIR antibody 67516-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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